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Takara is here to help, how to determine the lentiviral/retroviral proviral integration site!

 Last Update: 2021-08-04
 Source: Internet
 Author: User

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dna sequencing gel

cell gene therapy

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Lentivirus and retroviral vectors are widely used in the field of life science research as powerful tools for introducing foreign genes into target cells

The location of the integration site has an important impact on the expression of the transgene and the phenotype of the host cell, and may even cause key gene mutations or activation of proto-oncogenes, which may increase the risk of malignant tumors

Mature integration site analysis technology

Takara can respectively provide lentiviral and retroviral provirus integration site analysis kits, using genome walking technology to obtain lentiviral/retroviral provirus integration site fragments in the genome of transduced cells, and subsequent expansion Sequencing and analysis of the amplified product can confirm the chromosomal location of the integration site

product name

Code No.

Applicable virus type

Lenti-X™ Integration Site Analysis Kit

631263

Lentivirus

Retro-X™ Integration Site Analysis Kit

631467

Retroviral

The genomic DNA of HT1080 cells after lentiviral integration was extracted, and the genomic DNA was digested with three different blunt-end cutting restriction enzymes, DraI, SspI, and HpaI, and linkers were connected to construct 3 virus integration libraries

T cell therapy research solutions

Takara can provide a more complete solution for T cell transformation and cultivation, and help T cell therapy research.

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