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Recently, Plant Biotechnology Journal published online the research titled "Single-cell trans"criptome reveals differentiation between adaxial and abaxial mesophyll cells in Brassica rapa" (single-cell transcriptome sequencing reveals the differentiation of mesophyll cells on the dorsal and ventral surfaces of Chinese cabbage)
.
At the single-cell level, the palisade histiocytes on the ventral surface of the leaf and the spongy histiocytes on the back surface were distinguished, and the differentiation of these two types of cells was revealed at the same time, which is an important progress in the study of the formation mechanism of Chinese cabbage leaf balls
.
.
At the single-cell level, the palisade histiocytes on the ventral surface of the leaf and the spongy histiocytes on the back surface were distinguished, and the differentiation of these two types of cells was revealed at the same time, which is an important progress in the study of the formation mechanism of Chinese cabbage leaf balls
.
Cabbage vegetables belong to the Brassica genus of the cruciferous family, and are important leafy vegetable crops in China.
Uta dishes
, etc.
Mesophyll cells are not only the largest cell group in leaves, but also the main site of photosynthesis, including the palisade tissue on the ventral surface of the leaf and the spongy tissue on the back
.
The differentiation and differentiation of palisade tissue and spongy tissue cells are important for maintaining leaf morphology
.
In recent years, with the application of single-cell RNA sequencing technology, the differentiation between leaf palisade tissue and spongy tissue cells has been explored at the single-cell level, and its key regulatory genes have been identified, which is important for revealing the role of leaf development and leaf ball formation in cabbage vegetables.
Mechanisms are very important
.
However, very little is known about it
.
Uta dishes
, etc.
Mesophyll cells are not only the largest cell group in leaves, but also the main site of photosynthesis, including the palisade tissue on the ventral surface of the leaf and the spongy tissue on the back
.
The differentiation and differentiation of palisade tissue and spongy tissue cells are important for maintaining leaf morphology
.
In recent years, with the application of single-cell RNA sequencing technology, the differentiation between leaf palisade tissue and spongy tissue cells has been explored at the single-cell level, and its key regulatory genes have been identified, which is important for revealing the role of leaf development and leaf ball formation in cabbage vegetables.
Mechanisms are very important
.
However, very little is known about it
.
The team constructed a single-cell transcriptome map of Chinese cabbage leaves by single-cell transcriptome sequencing, which contained 16,055 high-quality cells, covering 8 cell types, namely mesophyll cells, epidermal cells, vascular cells, vascular bundle sheaths, guards cells, proliferating cells, phloem and xylem
.
The team further used an improved tape-sandwich method to isolate leaf palisade tissue and sponge tissue.
Through transcriptome analysis and in situ hybridization, a batch of new marker genes was discovered and verified, thereby linking the palisade cells on the ventral surface and the sponge cells on the back surface.
differentiated at the single-cell level
.
Single-cell transcriptome analysis revealed a distinct functional difference between the two, that is, palisade cells mainly perform photosynthesis, while sponge cells mainly respond to external environmental stimuli
.
It was also found that palisade cells are rich in a large number of ribosomal protein-encoding genes, some of which have been shown to be involved in the establishment of leaf dorsal-ventral polarity in Arabidopsis thaliana
.
Interestingly, most of the known dorsoventral polarity genes were hardly expressed in mature leaves, but were specifically expressed at the initiation or early stages of leaf development
.
.
The team further used an improved tape-sandwich method to isolate leaf palisade tissue and sponge tissue.
Through transcriptome analysis and in situ hybridization, a batch of new marker genes was discovered and verified, thereby linking the palisade cells on the ventral surface and the sponge cells on the back surface.
differentiated at the single-cell level
.
Single-cell transcriptome analysis revealed a distinct functional difference between the two, that is, palisade cells mainly perform photosynthesis, while sponge cells mainly respond to external environmental stimuli
.
It was also found that palisade cells are rich in a large number of ribosomal protein-encoding genes, some of which have been shown to be involved in the establishment of leaf dorsal-ventral polarity in Arabidopsis thaliana
.
Interestingly, most of the known dorsoventral polarity genes were hardly expressed in mature leaves, but were specifically expressed at the initiation or early stages of leaf development
.
This study provides new insights into the complex process of leaf development and morphogenesis in Chinese cabbage and other Brassica crops
.
In addition, this study provides many cell-type-specific marker genes, which will facilitate the application of single-cell transcriptome sequencing technology in Chinese cabbage
.
The results are of great significance for analyzing the formation mechanism of Chinese cabbage leaf balls
.
.
In addition, this study provides many cell-type-specific marker genes, which will facilitate the application of single-cell transcriptome sequencing technology in Chinese cabbage
.
The results are of great significance for analyzing the formation mechanism of Chinese cabbage leaf balls
.
Guo Xinlei and associate researcher Liang Jianli, who have graduated from the vegetable molecular design breeding team, are the co-first authors, and researchers Wu Jian and Wang Xiaowu are the corresponding authors of the paper
.
This research was supported by the National Key R&D Program (2021YFF1000101), Fundamental Research Funds (Y2020PT21), and Innovative Engineering Programs
.
.
This research was supported by the National Key R&D Program (2021YFF1000101), Fundamental Research Funds (Y2020PT21), and Innovative Engineering Programs
.
Article link: https://doi.
org/10.
1111/pbi.
13919
org/10.
1111/pbi.
13919
