Scientists have discovered a new diagnostic marker for bovine TB

recently, the veterinary public health safety and management team of the Beijing Institute of Livestock and Veterinary Medicine of the Chinese Academy of Agricultural Sciences found that LTA, IL-8 and other new molecular markers can be used for the diagnosis of bovine tuberculosis, which is of great significance to the early diagnosis and prevention and control of bovine tuberculosis. The findings were published online in Frontiers in Veterinary Science.
Zhu Hongfei, a researcher at the Beijing Institute of Livestock and Veterinary Medicine of the Chinese Academy of Agricultural Sciences, said that bovine tuberculosis is a chronic expendable zoonotic infection caused by mycobacteria infection in cattle, which can infect people, cows, a variety of domestic animals and wild animals. A person may also contract the disease by drinking incompletely disinfected milk through the digestive tract or by inhaling polluted air through the respiratory tract. At present, the world's prevention and control of bovine tuberculosis is mainly based on the "test-kill" strategy, the skin pervert reaction test and IFN-γ release test is the OIE recommended diagnosis of bovine tuberculosis, but neither can distinguish the infection period of TB cattle, and the elimination of positive cattle will also cause corresponding economic losses.
In this way, screening new and effective molecular targets, developing more effective diagnostic reagents for bovine tuberculosis, and accurately and timely detecting and eliminating TB cattle, especially sterilized TB cattle, are of great significance to the prevention and control of bovine TB, animal husbandry development and public health security in China.
team found that about 24.87 percent of TB cattle were in the sterilization phase and that mycobacteria was detected in their nasal secretions. Using RNA-Seq technology to analyze the transcriptional spectrum of exostatic blood lymphocytes under specific antigen stimulation or non-stimulation conditions, the transcriptional spectrum of tuberculosis and extraterrescional blood lymphocytes in different infection states was found to have significant differences, which initially clarified the different infection status of TB cattle. Immune response, found that LTA, IL-8, BCL2 and CHI3L1 have the potential to act as molecular diagnostic markers of bovine TB, where IL-8 and LTA can distinguish between TB infected cattle and healthy cattle, and BCL-2 and CHI3L1 can distinguish between sterilization and non-sterilization period of TB cattle.
the study provides reference data for the development of new bovine TB diagnostic agents and the mechanism of bovine TB disease. (Source: Zhang Qingdan, China Science Daily)
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