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    Home > Biochemistry News > Plant Extracts News > Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

    Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

    • Last Update: 2021-03-11
    • Source: Internet
    • Author: User
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    Phytoplasmas are routinely detected by nucleic acid-based techniques. These approaches rely on enriched phytoplasma
    DNA
    extracts of good quality, following labor intensive and time-consuming purification protocols. Here we describe a very rapid, specific, sensitive, and reliable method for flavescence dor�e phytoplasma detection, based on real-time Taqman

    reverse transcription-
    PCR
    of the 16S rRNA. The protocol is particularly useful for large-scale screening of vineyards and nurseries, pathogen surveys, and field epidemiological studies.
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