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Recently, the innovation team of "Agricultural Product Quality and Safety Risk Assessment" of the Institute of Agricultural Quality Standards and Detection Technology of the Chinese Academy of Agricultural Sciences, together with the relevant team of Beijing University of Chemical Technology, invented a new TYPE of DNA nanofluorescence probe designed by molecular simulation, which has high sensitivity and high specificity, avoids the false positive signal caused by multi-channel fluorescence mutual interference, and has been successfully applied
in the study of the regulation ability and mechanism of allicin on the redox level of cells and tumor-bearing mice 。 The relevant achievements were published in Nano Today (IF:18.
963), an international authoritative journal in the field of nanotechnology, and have been authorized by the national invention patent (ZL202010544295.
4).
in the study of the regulation ability and mechanism of allicin on the redox level of cells and tumor-bearing mice 。 The relevant achievements were published in Nano Today (IF:18.
963), an international authoritative journal in the field of nanotechnology, and have been authorized by the national invention patent (ZL202010544295.
4).
Allicin is an organic sulfur compound formed by the decomposition and synthesis of alliin catalyzed by alliinase in garlic, and has the nutritional activity
of regulating the redox effect of living organisms because of its unique chemical structure.
In the molecular reactions related to oxidative stress, the active expression level of the endonuclease APE1 of the body's depurine pyrimidine endonuclease WAS1 was closely correlated with the ROS content of reactive oxygen species, and the mechanism of orthogonal in situ reporting of ROS and APE1 was taken as the entry point
to study the relevant mechanism of allicin redox level regulation.
of regulating the redox effect of living organisms because of its unique chemical structure.
In the molecular reactions related to oxidative stress, the active expression level of the endonuclease APE1 of the body's depurine pyrimidine endonuclease WAS1 was closely correlated with the ROS content of reactive oxygen species, and the mechanism of orthogonal in situ reporting of ROS and APE1 was taken as the entry point
to study the relevant mechanism of allicin redox level regulation.
To this end, the research team used molecular simulation tools to innovatively design a dual-channel fluorescent probe based on DNA nanostructures for in vitro and in vivo imaging to explain the mechanism
of action of allicin in regulating redox levels.
Molecular simulation assisted by the elimination of false-positive signals due to poor interactions between multiple fluorophores, improving the sensitivity and specificity of the probe; The nucleophilic target NLS was introduced into the probe to help it enter the nucleus efficiently, and the expression
of ROS and APE1 in subcellular structures was elucidated.
Quantitative and localization studies of ROS and APE1 expression in living cells and tumor-bearing mice showed that elevated ROS led to APE1 overexpression, mainly due to damage to the accumulated
bases in genomic DNA and mtDNA.
The fluorescent probe was applied to the study of the activity of the natural product allicin, and the results showed that allicin can effectively reduce cellular oxidative stress and protect cells from oxidative stimulation
.
of action of allicin in regulating redox levels.
Molecular simulation assisted by the elimination of false-positive signals due to poor interactions between multiple fluorophores, improving the sensitivity and specificity of the probe; The nucleophilic target NLS was introduced into the probe to help it enter the nucleus efficiently, and the expression
of ROS and APE1 in subcellular structures was elucidated.
Quantitative and localization studies of ROS and APE1 expression in living cells and tumor-bearing mice showed that elevated ROS led to APE1 overexpression, mainly due to damage to the accumulated
bases in genomic DNA and mtDNA.
The fluorescent probe was applied to the study of the activity of the natural product allicin, and the results showed that allicin can effectively reduce cellular oxidative stress and protect cells from oxidative stimulation
.
This work demonstrates a widely available strategy to construct nanostructured molecular probes that can image multiple targets in living cells and experimental animals for in-depth understanding of molecular interaction mechanisms in living cells and living organisms, and also provide a theoretical basis and important technical means
for the efficacy research and innovation of allicin, product processing and development.
for the efficacy research and innovation of allicin, product processing and development.
This research has been funded
by the National Characteristic Vegetable Industry Technology System, the National Agricultural Product Quality and Safety Risk Assessment, and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.
The paper was written by Pan Yecan, a doctoral student of our institute, and Qian Yongzhong, a researcher, and Professor Su Xin of Beijing University of Chemical Technology, as co-corresponding authors
.
by the National Characteristic Vegetable Industry Technology System, the National Agricultural Product Quality and Safety Risk Assessment, and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.
The paper was written by Pan Yecan, a doctoral student of our institute, and Qian Yongzhong, a researcher, and Professor Su Xin of Beijing University of Chemical Technology, as co-corresponding authors
.
Original link: https://doi.
org/10.
1016/j.
nantod.
2022.
101573
org/10.
1016/j.
nantod.
2022.
101573
