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| Researchers bred a new type of high-oil and high-efficiency haploid induction line |
Recently, the team of Professor Chen Shaojiang of China Agricultural University published a research paper online in Acta Agronomica Sinica (English edition).
By simultaneously selecting maize haploid induction efficiency and kernel oil content, a new high-oil and high-efficiency maize haploid induction line CHOI4 was developed.
.
Double seeding technology is one of the core technologies of maize breeding, which mainly includes three key links: haploid induction, haploid identification and haploid doubling
.
Among them, the induction of maize haploids depends on the induction line
In 2003, Chen Shaojiang proposed a method to identify haploids by using grain oil content and successfully developed automated identification equipment
.
On this basis, if the induction rate and oil content of the induction system are further increased, not only can the induction efficiency of haploid be improved, but also the efficiency and accuracy of identification can be improved, so as to achieve the purpose of saving cost and increasing efficiency
The researchers used the previously bred haploid inducible line CAU2 and the Beinong University High Oil Population (BHO) as the donor parents for the selection of the new inducible line, and performed molecular marker-assisted selection of the zmpla1 gene in the F2 and F3 generations
.
Through the selection of grain oil, induction efficiency, pollen amount, comprehensive agronomic traits and other indicators, a new induction line was bred with an average efficiency of 15.
Related paper information: https://doi.
https://doi.
org/10.
1016/j.
cj.
2021.
07.
009
