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Extracellular vesicles (EVs) are cells secreted by cells with lipid bilayer structures, carrying biologically active molecules such as DNA, RNA, proteins and lipids, and are important carriers of intercellular communication, which can transport various active biomolecules from EVs to related recipient cells
On September 6, 2022, small Methods magazine published online the findings of a study
Figure 1 Article information
This study proposes a high-throughput sequencing method for EVs based on the 10x Genomics platform, which explores
Analysis of the EV transcriptome found that the average number of mRNA genes contained in a single EV was 52, with different EVs ranging
This study reveals the transcriptome characteristics and heterogeneity of EVs at the single EV level for the first time through a high-throughput method, refreshing people's understanding of RNA contents in individual EVs and providing an important basis
Figure 2 Single EV transcriptome analysis process based on single-cell sequencing technology
The research has been funded by the National Key R&D Program, the National Natural Science Foundation of China, the Natural Science Foundation of Hubei Province and the Outstanding Doctor program of Zhongnan Hospital, and has been strongly supported
Prof.
References: Luo T, Chen SY, Qiu ZX, Miao YR, Ding Y, Pan XY, Li Y, Lei Q, Guo AY.
Full-text links: https://onlinelibrary.
