Regulation of JNK signaling pathway on inhibition of Eca-109 cell proliferation by grape seed extract
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Last Update: 2014-08-12
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Source: Internet
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Author: User
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Recently, researchers from the school of basic medicine of Lanzhou University and the Key Laboratory of preclinical research of new drugs in Gansu Province published a paper to explore the regulatory effect of c-jun amino terminal kinase (JNK) signal pathway on the inhibition of grape seed extract on the proliferation of Eca-109 cells It is suggested that JNK signaling pathway is involved in the regulation of grape seed extract on the inhibition of Eca-109 cell proliferation Inhibition of JNK activity can reduce the inhibition of proliferation and apoptosis induction of Eca-109 cell by grape seed extract This article was published in the Journal of Lanzhou University (Medical Edition), the first issue in 2014 Eca-109 cells were cultured in vitro, treated with different concentrations of grape seed extract and JNK specific inhibitor sp600125 Cell proliferation was detected by thiazole blue colorimetry, cell morphology was observed under inverted phase contrast microscope, apoptosis was observed by annexin V / propidium iodide double labeling, and expression of phosphorylated JNK protein was detected by Western blot The inhibition rate of proliferation and apoptosis of Eca-109 cells were significantly increased with the increase of phosphorylated JNK protein level, which was time-dependent and dose-dependent Sp600125 could reduce the inhibition rate of proliferation and apoptosis of Eca-109 cells.
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