Progress in the research of active components of natural drugs

Traditional Chinese medicine and natural medicine are important parts of Chinese medicine system Their chemical components are very complex It has been the key content of interdisciplinary innovation of pharmaceutical chemistry and other disciplines to find and prepare the seedling molecules with strong biological activity The team of Yang Junli, Key Laboratory of plant resources chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, aimed at the serious problems of repeated separation of active ingredients of natural drugs, lack of targeted preparation technology, long activity screening cycle, etc., established a new strategy for rapid discovery of natural active compounds by using capillary electrophoresis, colorimetric / fluorescence analysis and other technologies and methods Using the effect of dopamine (DA) on the color and the ratio of fluorescence emission spectrum of Si NPs solution, the researchers developed a new method for the sensitive analysis of tyrosinase activity and the screening of inhibitors / activators In this work, tyramine was used as substrate and tyrosinase as catalyst Tyramine generated Da through enzymatic reaction, which further triggered the change of color and fluorescence spectral ratio of Si NPs solution The detection limit (LOD) is 0.14 U / ml Using this method, the team successfully screened out the tyrosinase activator C7 from natural sources Compared with 8-MOP, the IC50 value of C7 activity is one order of magnitude higher This study provides a new lead compound for the development of tyrosinase activator drugs Relevant research results are published in talanta, 2019197113 – 121 Researchers have also developed a variety of new analytical methods around the new strategy of discovering active components of natural drugs Some research results have been published in food chemistry, 2018259245 – 250; sensors and actuators B, 2018260808 – 815 and other journals In addition, with the advantages of rapid and sensitive separation and analysis of capillary electrophoresis, target enzyme reaction and natural drug inhibitor screening are integrated The researchers reviewed and introduced the new progress of capillary electrophoresis enzyme inhibitor screening, providing a new idea for rapid and accurate discovery of natural active compounds The results were published in electrophoretic journal electrophoresis, 2019, DOI: 10.1002/elps.201900013; electrophoresis, 2018, 392117 – 2124; electrophoresis 2017, 381685 – 1692.