Progress in research on bovine and human embryonic stem cells in China

Introduction: new page 1 the national major scientific and technological project "Research on bovine and human embryonic stem cells" undertaken by researchers of northwest agricultural and Forestry University of science and technology has recently passed the expert identification organized by the Ministry of education Experts believe that this achievement as a whole has reached the international advanced level Xku in 1993, northwest agricultural and Forestry University of science and technology first carried out research on bovine embryonic stem cells in China In 1995, under the chairmanship of Professor Dou Zhongying, an expert in embryo engineering and doctoral supervisor, northwest agricultural and Forestry University of science and technology isolated and cloned bovine stem cells for the first time in China; in 1999, the highest number of bovine stem cells derived from the inner cell mass of bovine early embryos was transferred to 6 generations; in 2000, the highest number of bovine embryonic stem cells derived from bovine primordial germ cells was transferred to 15 generations, all of which were the highest in China Algebra The results of morphological observation, in vitro differentiation experiment and karyotype analysis showed that the cloned cells had the characteristics of bovine stem cells They obtained epithelial cells, chondroid cells and neuron like cells through differentiation experiments in vitro, and obtained bovine heart beating cell clusters for the first time in the world, and established the bovine embryonic cloning technology with bovine stem cells as nuclear donors, which laid a foundation for the isolation and cloning of bovine embryonic stem cells from cloned embryos In 1997, human stem cells were first isolated from northwest agricultural and Forestry University of science and technology, and cloned to 13 generations, which is the highest algebra in China After differentiation in vitro, epithelioid cells, neuron like cells, glial like cells and fat like cells were obtained In particular, on August 31, 2000, they obtained the human heart beating like cell clusters for the first time in the world, and established the human cell cloning embryo technology This technology laid a foundation for the study of human stem cells isolation and cloning with cloned embryos, and explored the suitable conditions for isolation and cloning of bovine and human stem cells Xku experts participating in the appraisal meeting believe that "Research on bovine and human embryonic stem cells" is a major cutting-edge course in life science in the world This research result has accumulated rich data for further in-depth study of bovine and human stem cells, which has important scientific significance and great application potential XkU