PRL/PRLR Can Advance Brain Glioma

Backgroundprolactin (prolactin, PRL) is a peptide hormone secreted from the pituitary front alve, and in addition to the pituitary front, organs that secrete PRL, including the breast, prostate, brain, immune cells, and skin, regulate the metabolism of the corresponding tissue through circumstantial/self-secretionIt was found that PRL, in addition to regulating lactation and reproductive function, was also involved in the development of a variety of tumorsStudies have shown that PRL, PRL receptor (PRL receptor) is highly expressive in breast and prostate cancer, and promotes tumor cell proliferation, angiogenesis, and immune chemotherapyIt has also been found that PRL and PRLR are expressed in glioblastoma (giloblastoma, GBM), and in vitro experiments have shown that PRL promotes GBM cell migration, activation, and over-expression PRL promotes GBM cell proliferationAntonela Sof?a Asad of the Institute of Biomedical Research at the Faculty of Medicine of the University of Buenos Aires, Argentina, and others studied the role of the PRL-PRLR pathway in GBM, the results of which were published in Scientific Reports in December 2019research methods
authors detect PRL/PRLR expression in cells in gliomas and tumor cells immersed in non-tumor brain essence through immunofluorescent and western blot methods, and detect PRL/PRLR attacks, proliferation, and The effects of chemotherapy resistance, and using the TCGA database, 530 cases of WHO II-III glioma (GII.-III) and 150 cases of GBM were analyzed for mRNA expression and clinical data of PRL, PRLR, matrix metalloproteinase-2 (MMP-2)resultsresults show that there are PRL and PRLR expression in GBM cells, PRL in the culture agent liquid, and PRL expression in tumor cells immersed in the substance of non-tumor brainWestern blot tests have shown that prLR's long, medium, and short subtypes are expressed in glioma cellsActivation of PRLR or overexpression PRL through PRL can promote the proliferation of glioma and inhibit the chemotherapy effect of cisplatin and tamoxaminePrLR inhibitors (PRLR-A) or overexpression PRLR-A can block the effects of PRLRTransfection of PRLR long and short subtype vectors can significantly increase resistance to chemotherapy, and PRLR-A blocks PRLR by activating MMP-2 to extend the scratch healing timeTCGA data analysis found that PRLR's level of expression between different levels of glioma specimens was close12% of GII.-IIIand 30% of GBM specimens can see PRL expression, the expression level of GBM is higher than GII.-III There was a significant correlation between mRNA levels in PRLR, PRL, MMP-2 in PRL-positive GBM specimens Survival analysis found that WHETR expression or PRLR expression level softened no effect on the survival curve of all levels of glioma patients, and further analysis suggested that PRL expression positive or PRLR overexpression reduced the long-term survival rate of patients, but no statistical difference the authors analyzed gender stratification of PRL differences between male and female patients It was found that there was no difference in PRLR expression between different genders and levels of glioma, but there was no correlation between PRL and PRLR expression in female GBM patients, while there was no correlation in male patients Median survival was shorter (10.5m: 26.7m; p0.05) in male GBM patients compared to the low expression group (10.5m: 26.7m; p0.05), and in male GII-III patients, median survival was shorter (37.89m: 100m; p.05 m In female GII-III patients, PRLR expression was unrelated to prognosis; in female GBM patients, the median survival of PRL/PRLR low group and PRL/PRLR high group was 6.6m:25.9m, but there was no statistical difference (p-0.06) conclusions authors note that this study is the first to reveal that the PRL-PRLR pathway has the effect of promoting the migration, activation and proliferation of GBM cells, and that PRL/PRLR expression is associated with prognosis and gender-related Therefore, PRLR is a potential target for GBM therapy, and gender influenceshould should be paid attention to in GBM treatment.