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for diploid potato hybrid breeding.
The relevant research results were published in the Journal
of Biotechnology (aBIOTECH).
The traditional method of obtaining diploid potato inbred lines generally requires multi-generation self-inbreeding, long cycle and low efficiency, while haplo species can achieve pure line creation only through haploid induction and doubling two generations, which significantly accelerates the breeding process and efficiency of inbred lines, which is a common key technology
in agricultural biological breeding.
in agricultural biological breeding.
The researchers combined with FAST-Red fluorescent screening markers through the CRISPR/Cas9 system to construct a biallele mutant
of the StDMP gene.
On this basis, nearly 100% homozygous diploid potato material
was obtained by hybridization, fluorescence screening, molecular marker identification, flow cytometry determination, and colchicine doubling.
Compared with the traditional self-inbred method, this method does not have to overcome the structural barriers of potato inbred incompatibility, and greatly shortens the breeding cycle, which can be completed in about 8 months, which will provide a new solution
for rapid potato breeding.
of the StDMP gene.
On this basis, nearly 100% homozygous diploid potato material
was obtained by hybridization, fluorescence screening, molecular marker identification, flow cytometry determination, and colchicine doubling.
Compared with the traditional self-inbred method, this method does not have to overcome the structural barriers of potato inbred incompatibility, and greatly shortens the breeding cycle, which can be completed in about 8 months, which will provide a new solution
for rapid potato breeding.
The project was supported
by the National Natural Science Foundation of China, the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences, and the Guangdong Provincial Major Project for Basic and Applied Basic Research.
by the National Natural Science Foundation of China, the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences, and the Guangdong Provincial Major Project for Basic and Applied Basic Research.
Original link: