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Plant Regeneration Methods for Rapid Generation of a Large Scale Ds Transposant Population in Rice

 Last Update: 2020-11-07
 Source: Internet
 Author: User

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To mutagenize rice genomes, a two-element system is utilized. This system comprises an immobile
Ac
element driven by the CaMV 35S promoter, and a gene trap
Ds
carrying a partial intron with alternative splice acceptors fused to the GUS coding region. Rapid, large-scale generation of a
Ds
transposant population was achieved using a plant regeneration procedure involving the tissue culture of seed-derived calli carrying
Ac
and
Ds
elements. During tissue cultures,
Ds
mobility accompanies changes in methylation patterns of a terminal region of
Ds
, where over 70 % of plants contained independent
Ds
insertions. In the transposon population, around 12 % of plants expressed GUS at the early seedling stage. A flanking-sequence-tag (FST) database has been established by cloning over 19,968
Ds
insertion sites and the
Ds
map shows relatively uniform distribution across the rice chromosomes.

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