Peking University team: Evaluation of colorectal cancer organoid culture system by single-cell sequencing system

Genome Biology [IF:1583] ① The tumor tissues and adjacent normal tissues of 7 colorectal cancer patients were included, and the organoids were cultured in vitro and single-cell transcriptome sequencing was performed;② The tumor organoids can accurately reflect the gene expression and gene mutation of the corresponding cancer cells in vivo and DNA methylation and other key features;③ Normal intestinal epithelial organoids showed some tumor-like characteristics at the transcriptome level, but maintained normal genome and DNA methylome characteristics in the culture system;④ Two types of Culture systems are effective in maintaining key characteristics of tumor cells in vivo, but conditioned media are superior to chemically defined media for long-term growth of tumor organoi. Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis04-28, doi: 11186/s13059-022-02673-3[Editor's comment] Colorectal cancer is one of the most common cancers in the wor. Today, researchers The use of patient tumor tissue to construct organoid systems has become a common method to study the molecular mechanism of tumorigenes. Recently, Peking University Tang Fuchou and Peking University Third Hospital Fu Wei and his team published their latest research in Genome Biolo. They collected tumor tissues and adjacent normal tissues from 7 colorectal cancer patients, and constructed corresponding organoid models through in vitro cultu. Based on transcriptome, genome and DNA methylome, it was found that tumor organoids in two existing culture systems can better maintain the key biological characteristics of cancer cells in patients, and conditioned medium is superior in long-term culture of tumor organoids in a chemically defined medi. In conclusion, this study provides new insights into the systematic evaluation of colorectal cancer organoid culture systems and the analysis of similarities and differences between organoids and cancer cells in vi.   Disclaimer: This article only represents the author's personal opinion and has nothing to do with China Probiotics Netwo. Its originality and the text and content stated in the text have not been verified by this site, and this site does not make any guarantee or commitment to the authenticity, completeness and timeliness of this text and all or part of its content and te. Readers are only for reference and please Verify the relevant content yourse. Copyright Notice Some articles reproduced on this site are not original, and their copyright and responsibility belong to the original auth. All reprinted articles, links and pictures on this website are for the purpose of conveying more information, and the source and author are clearly indicat. Media or individuals who do not wish to be reprinted can contact us for infringing information that can provide sufficient eviden. , bio149 will be deleted within 12 hours after confirmati. Users are welcome to submit original articles to 86371366@. com, which will be published on the homepage after review, and the copyright and responsibility of the articles belong to the send.

Genome Biology① The tumor tissues and adjacent normal tissues of 7 colorectal cancer patients were included, and the organoids were cultured in vitro and single-cell transcriptome sequencing was performed;② The tumor organoids can accurately reflect the gene expression and gene mutation of the corresponding cancer cells in vivo and DNA methylation and other key features;③ Normal intestinal epithelial organoids showed some tumor-like characteristics at the transcriptome level, but maintained normal genome and DNA methylome characteristics in the culture system;④ Two types of Culture systems are effective in maintaining key characteristics of tumor cells in vivo, but conditioned media are superior to chemically defined media for long-term growth of tumor organoi.

Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis04-28, doi: 11186/s13059-022-02673-3[Editor's comment] Colorectal cancer is one of the most common cancers in the wor.

Today, researchers The use of patient tumor tissue to construct organoid systems has become a common method to study the molecular mechanism of tumorigenes.

Recently, Peking University Tang Fuchou and Peking University Third Hospital Fu Wei and his team published their latest research in Genome Biolo.

They collected tumor tissues and adjacent normal tissues from 7 colorectal cancer patients, and constructed corresponding organoid models through in vitro cultu.

Based on transcriptome, genome and DNA methylome, it was found that tumor organoids in two existing culture systems can better maintain the key biological characteristics of cancer cells in patients, and conditioned medium is superior in long-term culture of tumor organoids in a chemically defined medi.

In conclusion, this study provides new insights into the systematic evaluation of colorectal cancer organoid culture systems and the analysis of similarities and differences between organoids and cancer cells in vi.

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Genome Biology① The tumor tissues and adjacent normal tissues of 7 colorectal cancer patients were included, and the organoids were cultured in vitro and single-cell transcriptome sequencing was performed;② The tumor organoids can accurately reflect the gene expression and gene mutation of the corresponding cancer cells in vivo and DNA methylation and other key features;③ Normal intestinal epithelial organoids showed some tumor-like characteristics at the transcriptome level, but maintained normal genome and DNA methylome characteristics in the culture system;④ Two types of Culture systems are effective in maintaining key characteristics of tumor cells in vivo, but conditioned media are superior to chemically defined media for long-term growth of tumor organoi.

Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis04-28, doi: 11186/s13059-022-02673-3[Editor's comment] Colorectal cancer is one of the most common cancers in the wor.

Today, researchers The use of patient tumor tissue to construct organoid systems has become a common method to study the molecular mechanism of tumorigenes.

Recently, Peking University Tang Fuchou and Peking University Third Hospital Fu Wei and his team published their latest research in Genome Biolo.

They collected tumor tissues and adjacent normal tissues from 7 colorectal cancer patients, and constructed corresponding organoid models through in vitro cultu.

Based on transcriptome, genome and DNA methylome, it was found that tumor organoids in two existing culture systems can better maintain the key biological characteristics of cancer cells in patients, and conditioned medium is superior in long-term culture of tumor organoids in a chemically defined medi.

In conclusion, this study provides new insights into the systematic evaluation of colorectal cancer organoid culture systems and the analysis of similarities and differences between organoids and cancer cells in vi.

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Genome Biology[IF:1583]① The tumor tissues and adjacent normal tissues of 7 colorectal cancer patients were included, and organoids were cultured in vitro and single-cell transcriptome sequencing was performed;② Tumor organoids can accurately reflect the presence of corresponding cancer cells in vi.

Key characteristics of gene expression, gene mutation and DNA methylation;③ Normal intestinal epithelial organoids show some tumor-like characteristics at the transcriptome level, but maintain normal genome and DNA methylome in the culture system Characteristics;④ Both culture systems are effective in maintaining key characteristics of tumor cells in vivo, but conditioned medium is superior to chemically defined medium in long-term culture of tumor organoi.

Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis04-28, doi: 11186/s13059-022-02673-3[Editor's comment] Colorectal cancer is one of the most common cancers in the wor.

Today, researchers The use of patient tumor tissue to construct organoid systems has become a common method to study the molecular mechanism of tumorigenes.

Recently, Peking University Tang Fuchou and Peking University Third Hospital Fu Wei and his team published their latest research in Genome Biolo.

They collected tumor tissues and adjacent normal tissues from 7 colorectal cancer patients, and constructed corresponding organoid models through in vitro cultu.

Based on transcriptome, genome and DNA methylome, it was found that tumor organoids in two existing culture systems can better maintain the key biological characteristics of cancer cells in patients, and conditioned medium is superior in long-term culture of tumor organoids in a chemically defined medi.

In conclusion, this study provides new insights into the systematic evaluation of colorectal cancer organoid culture systems and the analysis of similarities and differences between organoids and cancer cells in vi.

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