Own a TA to do more experiments with less...

TA has long been recognized and trusted by everyone, such as "normal cell shape", "fast cell growth rate", "adherent cells adhere well", and "cells are not easily contaminated"
.
That’s the point.
Which cells are our Sigma serums popular? Let’s just look at the pictures: Cell type research purpose Human bone marrow mesenchymal stem cell tumor research, stem cell research U87MG (human glioblastoma cells) ) tumor research, drug development Caov-3 (human ovarian cancer cells) tumor research, drug development RPE1 (human retinal pigment epithelial cells) metabolic research PANC-1 (human pancreatic cancer cells) tumor research BxPC-3 (human pancreatic cancer in situ) cell) tumor research SNU-387/182 (human liver cancer cells) tumor research Huh-7 (human liver cancer cells) tumor research NK92 (human malignant non-Hodgkin lymphoma patients NK cells) tumor, immunology research, cell therapy 16HBE ( Human bronchial epithelioid cells) immunological research T lymphocyte cell therapy Reh (human acute lymphoblastic leukemia cells) tumor, immunological research U937 (human histiocytic lymphoma cell) tumor, immunological research HeLa (human cervical cancer cell) tumor Research JeKo-1 (human mantle cell lymphoma cells) tumor research A549 (human non-small cell lung cancer cells) tumor research 293T (human embryonic kidney cells) drug development / neurological research MC38 (mouse colon cancer cells) tumor research, drugs Development of SP2/0-Ag14 (mouse myeloma cells) drug development, IVD antibody development Research, drug development C2C12 (mouse myoblasts) stem cell research B16 (mouse melanoma cells) tumor research, drug development mESC (mouse embryonic stem cells) stem cell research Hepa 1-6 (mouse liver cancer cells) tumor research L929 ( Mouse fibroblasts) metabolic research EMT6 (mouse breast cancer cells) tumor research NRK-52E (rat renal tubular epithelial cells) metabolic research CHO (hamster ovary cells); CHO-K1 tumor research, drug development, immunology research MDCKII-MDR1 (canine kidney epithelial cells) metabolism research, drug development Vero E6 (African green monkey kidney cells) immunological research, drug development pig primary bone marrow mesenchymal stem cell stem cell research Many cell types have been verified, if in If you have the above cell types in your research, please come to me [Miss Liu WeChat ID: emma891021]! After sharing the good things, let's talk about the things behind it
.

When it comes to feeding cells, it has to be said that the most important supplement in the cell culture medium is bovine serum (usually 2%-20%)
.

Bovine serum contains a variety of cell growth-promoting factors, adhesion-promoting factors, hormones and other active substances
.

Serum has obvious effects on cell attachment and protection, and can neutralize the toxicity of toxic substances, so that cells are not harmed.
Serum can also be used as a buffer to avoid various factors (including pH changes, proteolytic activity or heavy metals, endotoxicity).
etc.
) interfere with cell growth or produce toxic effects
.

As a key component of cell culture medium, bovine serum is difficult to be replaced by other substances, so ensuring the quality of bovine serum is a crucial link
.

The 2000 edition of "Quality Control Standards for Main Raw Materials and Excipients of Chinese Biological Products" put forward relatively strict standard requirements for the quality of bovine serum, mainly including the following six points: shape: light yellow clear and slightly viscous liquid, no hemolysis or foreign matter; serum The total protein content should be 3.
5%-5.
0% (w/v); no bacterial contamination, bacterial endotoxin ≤10EU/ml, no E.
coli, bacteriophage and mycoplasma contamination; no bovine diarrhea virus contamination in serum, Bovine diarrhea virus can contaminate live swine fever vaccine and infect vaccinated pigs; hemoglobin≤0.
02%(w/v)
.

The content of hemoglobin directly affects the activity of stimulating cell growth factors in serum
.

Serum high in hemoglobin, dark red in color, of poor quality; supports cell growth
.

The evaluation of the ability of serum to cell proliferation includes the determination of cell growth curve, cell doubling time and cloning rate
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There are many types of bovine serum, among which fetal bovine serum is of high quality
.

Because fetal bovine serum (FBS) is obtained from caesarean section fetal bovine, the fetal bovine has not been exposed to the outside world, and the serum contains the least components such as antibodies and complement that are harmful to cells
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The main components of FBS are proteins, polypeptides and hormones.
The proteins that have been found to be effective include albumin, α1 antitrypsin, transferrin, Ig antibodies, as well as different contents such as α2 macroglobulin, A1 and B1 lipids Protein, cholesterol, fibronectin, bilirubin and carotenoids
.

Due to the uncertain composition of fetal bovine serum, it is not possible to definitively identify which composition has the greatest effect on cell culture
.

Polypeptide may be the main cell-proliferating factor in fetal bovine serum, which has the effect of promoting the proliferation of fibroblasts and glial cells; the effect of hormones on cells is multi-faceted, for example, insulin can promote the uptake of glucose and amino acids by cells
.

In addition, studies have shown that the difference between the cell adhesion-related factors of different fetal bovine serum such as fibronectin (Fibronectin, Fn) and in vitro connexin (Vitronectin, Vn) has a great influence on the growth rate and cell viability of cells.
Therefore, it is speculated that the cell adhesion effect of fetal bovine serum may also be a key factor affecting the growth, differentiation, reproduction and apoptosis of adherent cells
.

There are many sources of fetal bovine serum, mainly China, North America, South America and Australia, among which the fetal bovine serum from Australia is recognized as a better and safer serum
.

However, due to factors such as the epidemic and the international situation, many brands of fetal bovine serum (such as Gibco) are in serious shortage.
Our company is the first-level agent of Merck and has purchased a large number of high-quality serum Gram Sigma Australian fetal bovine serum, available from stock (provided trial)
.

Product Description F8318 Fetal Bovine Serum, Australian Source F8687 Fetal Bovine Serum, Australian Source, gamma irradiated Sigma Fetal Bovine Serum Advantages 1Sigma Fetal Bovine Serum The materials used in the preparation of Sigma Fetal Bovine Serum are all from Australia.
None of the sites have passed through the mad cow disease (BSE) epidemic area, and the raw materials have passed strict standards and are collected from the non-Bluetongue virus (BTV) areas in Australia licensed by China.
Traceability; 2.
Sterile cardiac puncture for blood collection, 3 times of 0.
1μm sterile filtration; 3.
Serum spot treated with γ-ray irradiation is available, and the possibility of virus and foreign microorganism contamination in γ-ray-irradiated serum is reduced Millions of times, and the cell growth-promoting effect of serum is completely unaffected by irradiation; 4 Strict QC standards for multiple tests, including bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVDV), bovine Parvovirus (BPV), bluetongue virus (BTV), bovine adenovirus (BAdV), rabies virus (RABV), respiratory enterovirus (REO) and other virus detection, mycoplasma, endotoxin, hemoglobin, IgG content, cell growth verification And a number of physiological and biochemical tests to ensure that the products have reliable high quality
.

For more information, please add Dongrui Technology Miss Liu's WeChat ID: emma891021
.

Sigma fetal bovine serum (Cat.
No.
: F8687) participated in the research and published articles: 1.
Salidroside inhibits endothelial‑mesenchymal transition via the KLF4/eNOS signaling pathway.
Yongpan Huang et al.
Molecular medicine reports, 24(4) (2021-08-10 ).
2.
Ixazomib inhibits myeloma cell proliferation by targeting UBE2K.
Qingqing Wang et al.
Biochemical and biophysical research communications, 549, 1-7 (2021-03-02).
3.
Improved Differentiation Ability and Therapeutic Effect of miR-23a- 3p Expressing Bone Marrow-Derived Mesenchymal Stem Cells in Mice Model with Acute Lung Injury.
Peng Zhang et al.
International journal of stem cells, 14(2), 229-239 (2021-02-27).
4.
Hydrogen gas alleviates blood -brain barrier impairment and cognitive dysfunction of septic mice in an Nrf2-dependent pathway.
Yang Yu et al.
International immunopharmacology, 85, 106585-106585 (2020-05-25).
5.
Silencing circular RNA-ZNF652 represses proliferation and EMT process of renal carcinoma cells via raising miR-205.
Ludong Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 648-655 (2020-02-20) .
6.
Silencing UNC5B antisense lncRNA 1 represses growth and metastasis of human Colon cancer cells via raising miR-622.
Yan Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 60-67 (2019-12-20 ).
7.
Genipin, a natural AKT inhibitor, targets the PH domain to affect downstream signaling and alleviates inflammation.
Yanting Jiao et al.
Biochemical pharmacology, 170, 113660-113660 (2019-10-13).
6.
Silencing UNC5B antisense lncRNA 1 represses growth and metastasis of human Colon cancer cells via raising miR-622.
Yan Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 60-67 (2019-12-20) .
7.
Genipin, a natural AKT inhibitor, targets the PH domain to affect downstream signaling and alleviates inflammation.
Yanting Jiao et al.
Biochemical pharmacology, 170, 113660-113660 (2019-10-13).
6.
Silencing UNC5B antisense lncRNA 1 represses growth and metastasis of human Colon cancer cells via raising miR-622.
Yan Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 60-67 (2019-12-20) .
7.
Genipin, a natural AKT inhibitor, targets the PH domain to affect downstream signaling and alleviates inflammation.
Yanting Jiao et al.
Biochemical pharmacology, 170, 113660-113660 (2019-10-13).