One of the three major challenges of the Great Breakthrough Human Proteome Project - protein identification of shear variants

Professor Zhang Gong of Jinan University developed the three-generation full-length translation group sequencing technology, broke through the protein product identification of shear variants of one of the three major problems of the human proteome project, found that there are thousands of mRNA shearing variants in human cells that have never been included in the database can be translated into proteins, and identified the characteristic peptides of some of the new shear variants at the protein level by mass spectrometry

Alternative splicing is widespread in eukaryotes, where a gene on a genome can produce multiple RNAs (called "variable spliced transcripts", AST), some of which can be translated into proteins

Professor Zhang Gong's team of Jinan University developed the three-generation full-length translation group sequencing technology, extracted the full-length mRNA (RNC-mRNA) being translated by two human cell lines, and successfully sequenced the translation group RNC-seq

The researchers were surprised to find that more than half of the shear variants identified in the sequencing of the third-generation full-length translation group (8954) could not be identified with extremely high throughput for second-generation sequencing, and 4525 of the shear variants were never included in the database, meaning that these proteins were never noticed by humans

This study establishes a relatively robust, replicable and commercialized proteome-level shear variant identification technology protocol, expands the map of the human proteome, and makes people realize that there are still a large number of shear variants of protein products that are not recognized

Professor Zhang Gong is the head of the Translation Omics Laboratory of Jinan University, a national outstanding youth and a top talent

Article:Detection of the Alternative Spliced Human Proteome Using Translatome Sequencing