On the safety of probiotics.

. Zhong Yu Sichuan Gaofu Ji Biotech Co., Ltd.
Although probiotics are well received, but with the expansion of the market size of probiotics, it is not possible to ignore the possible risk of disease-causing strains. The company contributes to the probiotic industry by analyzing the safety of probiotics through multi-aspect identification and experimentation.
, with more and more probiotic products on the market, the probiotic market appears to be in high demand. According to the "China Lactic acid bacteria market in-depth analysis report 2016-2019" published by New Thought Industry Research, the market size of lactic acid bacteria in China reached 66 billion yuan in 2015, and the market size expanded ninefold in 8 years.
are often defined as active microorganisms that, when ingested in sufficient quantities, are beneficial to the host's health. Probiotics have been widely used around the world, and although consumption is increasing, there are few negative reports. The probiotics commonly used, such as lactic acid bacteria (rod) bacteria, Bipolithia, etc. are considered to be part of a healthy human intestinal microbiome and are not harmful to the human body. Most lactic acid bacteria are food-grade microorganisms with long-term safe use conditions, especially lactic acid bacteria are considered safe (GRAS) strains. Based on sources, long-term history and a number of basic safety studies, on 22 April 2010, the General Office of the Ministry of Health issued the List of Bacteria Available for Food, which was gradually updated over the next few years to establish the basic line of defence for microbial safety in China. However, due to the diversity of microbial genes and metabolic complexity and other reasons, also does not rule out the list of strains may be disease-causing risk.
Gaofushi Biotech Co., Ltd. (hereinafter referred to as "Gaofuj Company") from 2003 began to specialize in lactic acid bacteria technology, according to traditional Fermented foods in China and healthy chinese people isolated excellent strains, the establishment of a local characteristics of the strain resource pool, collection and preservation of more than 1000 strains, through its unique "low temperature spray drying" process can save probiotics at room temperature. In the course of its development, The company attaches great importance to
folquality food safety
issues, especially in the safety of strains committed to the establishment of a sound set of probiotic safety evaluation methods and procedures. Here's a look at some of the research on the safety of the bacteria at Goforth for reference.
of the bacteria species and identification
The source of the probiotic species must be screening for traditional fermented foods with a long history of consumption and healthy human intestines isolated from various physiological indicators normal and without a history of genetic diseases. The source of the strain is the basis for the safety screening of strains, which need to be avoided by nature, especially new probiotic strains with no long history of safe use and probiotics in which the genus cannot guarantee safety. Gaofu remembers plant Lactobacillus 550/360, Lactobacillus acidophilus 11073 are screened separated in Sichuan traditional fermented kimchi, with thousands of years of edible history, so far there are no unsafe cases reported, in the source of the basic protection.
identification of probiotic strains in taxonomy is the basis for the evaluation of probiotic safety and the first step of safety evaluation. On the basis of the understanding of the genus characteristics of the strain, it is erbiscus to make preliminary judgments and predictions on the characteristics of the evaluated strain. Some probiotic products on the market do not have the details of the labeled strains, which not only related to the safety of probiotics, but also related to intellectual property rights and consumers' right to know and other issues. FAO-WHO recommends that the strain be tested first, followed by genetic identification using DNA/DNA hybridization, 16SRNA sequence determination and other reliable methods, and using DNA pulse field gradient electrophoresis (PFGE) to identify the strain. All probiotics produced commercially by Goforth are classified in strict accordance with this standard to ensure that they are within the scope of the List of Bacteria Available for Food promulgated by the State.
Harmful Metabolites Analysis
Probiotic metabolites are diverse, and most metabolites have been shown to be beneficial or beneficial to the human body, such as organic acids, lactic acid bacteria, polysaccharides, amino acids, peptides and other substances. But it is also important not to ignore the harmful products it may produce, such as nitrites, D-lactic acid, bioamines, aromatic amines, etc. Nitrites and aromatic amines are carcinogens that will have adverse effects when they enter the human body, so the determination of nitro reductase and anitrous reductase activity is a mandatory test for harmful metabolites of probiotics. A large number of D-lactic acid, bioamines will cause human poisoning, these two products must be selected probiotics can not be produced.
addition, hemolytic, lysogen, enterotoxins, endotoxins of strains are all items that need to be tested to avoid the potential risks of erythroblast destruction, bilior salt dissociasis, and intestinal mucosa shedding.
Gaofu note company separation screening plant Lactobacillus 550/360, after the relevant metabolites analysis, not only will not produce harmful metabolites, but also nitrite, aflatoxin, heavy metals and other harmful substances have a strong degradation effect, has been widely used in food, beverages and other fermentation industry.
antibiotic tolerance and infection capacity
with the widespread use of antibiotics, people have a deeper understanding of the potential harm caused by antibiotics. Many scientists also place their hopes on probiotics to replace antibiotics. As a microorganism, the determination of antibiotic resistance to probiotic strains is an important part of safety assessment. Scientists such as Salyers and Mathur have found that resistance transfer may occur between probiotics and pathogens in the gut.
The probiotic genome contains antibiotic resistance genes that are not a safety issue, as long as the gene is not transferred to other strains, so Goforth systematically screens every strain in the strain library for antibiotic resistance gene susceptivity to ensure that the drug-resistant gene or drug-resistant gene is located on the chromosome.
in the use of probiotics, the widespread concern is the ability to infect, especially the risk of sepsis. Adhesion is an important condition for probiotics to function, plant, and eliminate pathogenic bacteria, but strong adhesion may increase the strain's ability to infect in the host.
probiotics have been widely used in food processing for many years and have always been relatively safe. At present, cases of clinical infection involving lactic acid bacteria come from people in a state of illness, especially those with severe immunodeficiency and patients with myocarditis with abnormal heart valve function. However, studies have also shown that the adhesion of lactic acid bacteria associated with bacteremia is very low. To date, there is not enough evidence to suggest that the adhesion of the strain to the host tissue is directly related to the occurrence of lactic acid bacteria leading to bacteremia. The PROSAFE Forum in the European Union also does not include adhesion capacity as a project for probiotic safety evaluation.
, as a responsible probiotic manufacturer, will still use the adhesion capacity of the bacteria as an evaluation criterion, select the appropriate adhesion ability of the bacteria, reduce the potential risk of infection.
Ad animal experiments and human trials
are described in the previous in-body experimental projects, from a scientific point of view, before a probiotic is widely used, animal experiments and human clinical experiments must be carried out, in order to assess the strain's toxicology, re-position possibility, seriously ill patients and other safety levels.
safety is the top priority of the food industry, the current use of probiotics in healthy people is relatively safe, commercialized strains must go through a thorough safety study to be listed. After the product is listed, enterprises should also take corresponding responsibility for the characteristics of probiotics, the establishment of after-sales, the epidemiological investigation of adverse consumer reactions. Gaofu note company is such a
fell food safety
as its own probiotic company, adhering to the "strict screening, objective evaluation, people-oriented, after-sales monitoring" service attitude, for the probiotic industry to make a force.
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