New technology of IPS and ES cell screening by electric separation invented in Japan

According to Japanese media reports, the research team led by Professor Takeshi Miyata of Keio University invented a new technology that can simply separate the required ES cells and iPS cells from feeder cells by using the different response characteristics of cells when voltage is applied Compared with the previous trypsin separation method, this technology has the advantages of simple operation and no cell damage   Researchers arrange 50 20 μ m transparent electrodes on a 10 mm long and 5 mm wide circuit board in advance, then sprinkle the culture solution containing cells on the circuit board, and then apply 100000 Hz AC voltage to it Because of the different reactions of various cells, ES cells can be recovered to the designated container, while only feeding cells are left on the circuit board When there are about 10 million cells in 1 ml culture medium, it will take about 15-20 minutes to separate cells with this device Although the time of this method has not changed much compared with the previous "enzyme" separation method, because of its simple operation, it is not affected by the proficiency of operation technology, even beginners can complete the cell separation work stably The results show that the current separation rate is 95% The researchers say that the separation rate will continue to improve in the future According to relevant authorities, the discovery of this technology can improve the efficiency of acquiring IPS and ES cells in large quantities, which is of great significance to the development of regenerative medicine and new drugs with IPS and ES cells as the main body.