New coronavirus RNA does not have the ability to integrate into the host genome

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is an RNA virus in the Coronavirus family, which caused the outbreak of new coronary pneumonia in 2019 and a global pandemic

According to the type of genome to which the virus belongs, viruses are mainly divided into four categories, including dsDNA, ssDNA, dsRNA and ssRNA

Recently, the research team of the Chinese Academy of Sciences and the Chinese Academy of Medical Sciences jointly published an online article entitled "Comprehensive analysis of RNA-seq and whole genome sequencing data reveals no evidence for SARS-CoV-2 integrating into host genome" in Protein&Cell, revealing SARS -CoV-2 RNA does not have the ability to integrate into the host genome

http://doi.

First, the research team performed ribonucleic acid sequence analysis on 293T, Huh-7 and Calu-3 cells infected with the new coronavirus, and identified 347, 3107 and 4171 chimeras from 293T, Huh-7 and Calu-3 cells, respectively.

As a result, the researchers further obtained 132 conserved chimeric genes from all three cell lines and analyzed the types of RNA

Common chimeric genes in human ribonucleic acid and new coronavirus ribonucleic acid

Subsequently, the researchers further performed a whole genome sequencing parallel to the ribonucleic acid sequence on the new coronavirus infection samples

Whole genome sequencing of new coronavirus infection samples

Finally, in order to verify that the source of the chimeric events in the transcriptome sequencing results was the introduction of random connections during the library construction process, the researchers mixed the RNA of the neocoronavirus-infected cells and uninfected zebrafish embryos to construct an RNA library and further perform RNA sequencing

Chimeric events may come from library construction, but are not natural events

In short, this study ruled out the ability of the novel coronavirus RNA to integrate into the host genome through systematic analysis of transcriptome sequencing, whole genome sequencing, and mixed sample transcriptome sequencing

Reference materials:

[1]https://link.