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Nature Methods: A new technology that can perform detailed genetic analysis on resting human immune cells

 Last Update: 2022-01-10
 Source: Internet
 Author: User

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For the first time, LMU researchers have developed a method for detailed genetic analysis of resting human immune cells

CD4+ T cells are an important part of the immune system and play a key role in protecting the body against pathogens

Quiescent CD4+ T cells are hardly adapted to genetic manipulation, because existing methods usually assume cell division, Keppler explained

In the future, researchers will be able to eliminate individual genes and entire signaling pathways and analyze their functions

On this basis, they sought a second "knock-in" method, that is, inserting additional or slightly modified genes, such as the green fluorescent protein (GFP) gene

Article title

Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analyses

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