Nature found that fat cells produce key proteins that produce heat

5;">5;">“”，？，。，，，。，，。

5;">ATP，ADP，，。，，，。

5;">，《Nature》“Creatine kinase B controls futile creatine cycling in thermogenic fat”，——B（CKB），CKB。

5;">Researchers first used the isotope method to study the activity of creatine kinase in brown adipocytes and found that brown adipocytes showed strong creatine kinase activity.

And through quantitative proteomics, it was further confirmed that CKB is the most abundant creatine kinase in brown adipocytes.

5;">In order to further study the physiological regulation of CKB expression in adipocytes, the researchers exposed mice to a cold environment (6°C) and a high temperature environment (30°C), and found that in cold-exposed brown adipose tissue, CKB mRNA The significant increase in the level of CKB indicates that CKB participates in the thermogenesis process of brown fat cells.

5;">Studies have shown that the process of fat thermogenesis is regulated by cAMP and promoted through the β3-adrenergic pathway.

Researchers used pharmacological stimulation of β3-adrenergic receptors and found that the level of CKB mRNA increased significantly, indicating that CKB expression is downstream of the cAMP-mediated heat production pathway and regulates the production of CKB protein content.

5;">Next, the researchers used shRNAs to silence the expression of the CKB gene and found that knocking down CKB would not affect the differentiation of brown adipocytes, but would reduce the cell's oxygen consumption, indicating that CKB mainly regulates the degradation of mitochondrial ATP.

At the same time, knocking down CKB will not change the respiration of non-thermogenic cells, and creatine kinase other than CKB will not change the respiration of brown adipocytes, indicating that CKB selectively regulates the respiration of brown adipocytes.

5;">Subsequently, the researchers determined that the meaningless creatine cycle is a unique feature of thermogenic adipocytes, and verified that CKB, as the mitochondrial creatine kinase in adipocytes, is necessary for the meaningless creatine cycle.

By constructing mice that knock out the CKB gene, the researchers found that under a certain diet, the mice that knock out the gene accumulate more fat, are more likely to be obese, and have higher fasting blood sugar and insulin sensitivity.

5;">In general, this study shows that CKB is the most active of all creatine kinases in the process of fat cell heat production.

By regulating the production of CKB, it may be possible to reduce the complications caused by obesity.

5;">Reference materials:

5;">1.

Creatine kinase B controls futile creatine cycling in thermogenic fat

5;">2.

https:// style="text-align:justify;text-indent:2em;">5;">5;">"Every holiday season, you get three kilograms of fat", are you getting fat this year? Obesity is a state caused by excessive accumulation of fat in the body, usually caused by excessive food intake or changes in the body's metabolism.
The body’s fatness and thinness are determined by the number and size of fat cells.

5;">The meaningless creatine cycle means that mitochondrial creatine kinase transfers the high-energy phosphate bonds on ATP to creatine to generate creatine phosphate and ADP, and then phosphatase hydrolyzes creatine phosphate to regenerate creatine, which continues to participate in a new round of ineffective cycles.

process.

5;">Recently, researchers from McGill University in Canada published an article titled "Creatine kinase B controls futile creatine cycling in thermogenic fat" in the top journal "Nature", revealing the key protein that regulates the meaningless creatine cycle in fat cells— -Creatine kinase B (CKB), and verified the important role of CKB on fat cell heat production and metabolism.

5;">Researchers first used the isotope method to study the activity of creatine kinase in brown adipocytes and found that brown adipocytes showed strong creatine kinase activity.

And through quantitative proteomics, it was further confirmed that CKB is the most abundant creatine kinase in brown adipocytes.

5;">In order to further study the physiological regulation of CKB expression in adipocytes, the researchers exposed mice to a cold environment (6°C) and a high temperature environment (30°C), and found that in cold-exposed brown adipose tissue, CKB mRNA The significant increase in the level of CKB indicates that CKB participates in the thermogenesis process of brown fat cells.

5;">Studies have shown that the process of fat thermogenesis is regulated by cAMP and promoted through the β3-adrenergic pathway.
Researchers used pharmacological stimulation of β3-adrenergic receptors and found that the level of CKB mRNA increased significantly, indicating that CKB expression is downstream of the cAMP-mediated heat production pathway and regulates the production of CKB protein content.

5;">Next, the researchers used shRNAs to silence the expression of the CKB gene and found that knocking down CKB would not affect the differentiation of brown adipocytes, but would reduce the cell's oxygen consumption, indicating that CKB mainly regulates the degradation of mitochondrial ATP.
At the same time, knocking down CKB will not change the respiration of non-thermogenic cells, and creatine kinase other than CKB will not change the respiration of brown adipocytes, indicating that CKB selectively regulates the respiration of brown adipocytes.
Quantitative proteomics and subcellular localization further confirmed that CKB is widespread in mitochondria.

5;">Subsequently, the researchers determined that the meaningless creatine cycle is a unique feature of thermogenic adipocytes, and verified that CKB, as the mitochondrial creatine kinase in adipocytes, is necessary for the meaningless creatine cycle.
By constructing mice that knock out the CKB gene, the researchers found that under a certain diet, the mice that knock out the gene accumulate more fat, are more likely to be obese, and have higher fasting blood sugar and insulin sensitivity.
damage.
This shows that CKB can promote metabolic health.

5;">In general, this study shows that CKB is the most active of all creatine kinases in the process of fat cell heat production.
By regulating the production of CKB, it may be possible to reduce the complications caused by obesity.
(Biological Exploration)

5;">Reference materials:

5;">1.
Creatine kinase B controls futile creatine cycling in thermogenic fat

5;">2.
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