NAT GENET: CRISPR gene editing has potential carcinogenicity
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Last Update: 2020-05-29
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Source: Internet
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Author: User
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Cas9 is usually introduced into cell lines to achieve CRISPR-Cas9-mediated genome editingRecently, researchers explored the genetic and transcriptional consequences of Cas9 expression itselfa genetic expression spectrum analysis of 165 gene expression strains of human cancer cell lines and their Cas9 expression, the researchers found that after the introduction of Cas9, the p53 pathway improved, especially in the wild TP53 (TP53-WT) cell lineThis is confirmed at the messenger RNA and protein levelsin addition, an increase in DNA repair levels was observed in the cell line expressed in Cas9Genetic representation of 42 cell line pairs shows that the introduction of Cas9 leads to the emergence and expansion of p53-inactivated mutationsThis has been demonstrated in competitive experiments with the homologous TP53-WT and TP53-null (TP53-/-) cell linesfinally, Cas9's activity in TP53-WT was lower than in TP53-mutant cell lines, and Cas9-induced p53 pathway activation affected cell sensitivity to genetic and chemical interferencethese findings may have a broad impact on the correct use of CRISPR-Cas9-mediated genome editing
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