Nat Commun: The Mei Yang/Yu Peng team reveals the key process of mDia2 regulating bone marrow transplantation of hematopoietic stem cells.

!---- hematopoietic stem cells (HSPCs) are the "source" of blood cells in the body, mainly in the stem cell "niche" in the bone marrow.HSPCs have the ability to migrate, return home, and re-implante bone marrow, making bone marrow transplants a routine and effective clinical method for treating many malignant blood diseases.therefore, it is important to study the molecular signaling network in the process of hematopoietic stem progenitor cell transplantation in order to improve the efficiency of hematopoietic stem progenitor cells nesting and implantation., a team of pathologists from Northwestern University Feinberg School of Medicine recently published a research thesis entitled "Diaphanous-Conin mDia2 regulates beta2 integrins to control hematopoietic stem and progenitor cellgraften" in the journal Nature Communications ."this study revealed that Formin protein mDia2 promotes the activity of serum reactive factor SRF by regulating the synthesis of straight-chain myokine bundle F-actin, regulates the expression of beta-2 integrators, and eventually migrates forward to the transfer of hematopoietic stem progenitor cells during bone marrow transplantation to ensure normal implantation and amplification in mouse bone marrow.researchers first performed a functional analysis of HSPCs in mDia2 missing mice and found that their ability to fix plant was impaired in competitive bone marrow transplants, and that the "resting" state of stem cells was lost after a bone marrow transplant.through invivia technology, further found that mDia2 knock-out hSPCs are blocked inside the internal blood vessels of the bone marrow, unable to transfer the endothelial migration into the stem cell "snr".subsequent analysis showed that mDia2 deficiency led to a decrease in The polarization and adhesion of HSPCs, a decrease in SRF transcription activity in cells, and inhibited the expression of the beta-2 integrated gene.a step closer test, beta 2 integrated is a downstream target gene of the transcription factor SRF, and reexpression of SRF or beta-2 integrated in mDia2 missing HSPCs can "save" the defective phenotype of bone marrow transplantation.in short, the above studies reveal the molecular mechanism of mDia2 regulating the bone marrow transplant process of hematopoietic stem cells in mice, analyze the process of mDia2 regulating HSPCs transendic migration implantation, and open up a new direction for Formin protein to participate in stem cell biology as a cytoskeleton regulator.noteworthy, mDia2 protein also plays an important role in the development of red blood cells in mice, such as mDia2 involved in cytocytic division, regulating the "nucleation" of red blood cells.these studies show that mDia2 plays an important role in regulating the function of the blood system..