NAR Qi Ji team of Fudan University and the Chunce team of Jiangxi Agricultural University obtained the spatio-temporal map of orchid organ occurrence

NAR| spatio-temporal atlas of flower organ occurrence: Qi Ji's team from Fudan University and the Chunce team from Jiangxi Agricultural University revealed the spatio-temporal dynamic changes of gene regulation in orchid organ initiation and development

The development of flower organs has a complex molecular regulatory mechanism, and genes exercise specific and precise functions at different tissue and development stages, regulating a series of processes

A spatiotemporal atlas of organogenesis in the development of orchid flowers

(https://academic.

In the early stage of flower organ construction, it mainly underwent the process

Orchidaceae has a high degree of biodiversity and is one of the largest families of angiosperms, with 763 genera and more than 28,000 species

Figure 1.

A total of 4685 genes were identified in multiple cell populations of early flower primordial groups that were related to developmental time, showing selective high expression patterns

Figure 2.

Through the analysis of the development trajectory of the flower cover cells, it was found that the gene expression of the incochine cells of the No.
5 flower bud showed a high degree of consistency, while the stage of the No.
6 flower bud showed a certain expression heterogeneity (Figure 3
).
A total of 987 genes were detected in meristem cell populations, different cells in different stages of differentiation, and their expression levels were highly correlated with histological location, and were significantly enriched in related pathways such as protein synthesis, photosynthesis, and cell wall synthesis
.

Figure 3.
Basal fractional tissue cell populations are involved in the morphological completion of the trowel
sheet.

Compared with the overgart and lip flap, the development of anthers begins later and the process is more complex: from the small cell mass in bud 5 to the anther primordial and vegetative tissue in bud 6, to the pollen grains in buds 7 and 8, the apical tissue of the anther, the beak and the sticky plate (Figure 4).

The analysis of 120 tissue types further showed that the vegetative tissues of anthers tended to have similar gene expressions in the early stages, with fewer specifically expressed genes, but underwent rapid tissue differentiation and morphogenesis in the later stages of development
.

Figure 4.
Multiple gene pathways are involved in the identification of anther organs and the differentiation of
cell types.

Liu Chang of Fudan University is the first author
of the paper.
Professor Guo Chunce of Jiangxi Agricultural University and Researcher Qi Ji of Fudan University are co-corresponding authors
of the paper.
The research was funded
by the National Natural Science Foundation of China, the State Key Laboratory of Genetic Engineering of Fudan University, and the Innovative High-level Talents of Jiangxi Province.