Nano-hole detection method to confirm new crown infection more quickly and accurately

, Oct. 13 (Xinhua Mao Li) Israel University of Technology School of Biomedical Engineering said recently, the institute's researchers proposed a new coronavirus detection method, which is expected to pave the way for more accurate diagnosis of infected people. The new approach is currently in the process of commercialization with a view to serving the public as soon as possible.
In routine new coronavirus polymerase chain reaction (PCR) tests, professionals take swab samples from patients and process them with several chemical solutions, removing substances such as proteins and fats, and extracting only the RNA present in the sample, i.e. a mixture of personal genetic material and viral (if any) RNA. The RNA is then reversed into DNA using a reverse transcription PCR and the DNA is cycled and amplified, and if the sample contains a new coronavirus, it can usually be detected after 35 cycles.
, however, the PCR detection method is flawed. Mainly in the large sample body virus detection, with the increase in the number of samples, the chance of errors also increased. In addition, sometimes the amount of viral RNA is too small, which makes it difficult to detect and prone to omission.
by Professor Amit
, a professor at the Faculty of Biomedical Engineering. The researchers believe that using their original technique, which replaces large sample collections with special nano-holes in the material, to analyze individual molecules looking for viruses ensures a smaller sample size and greater accuracy. During the analysis, the molecules emit unique electrical signals as they pass through the electrical sensor. At the same time, in addition to the target molecules remain intact, other molecules will be removed, thereby improving the accuracy of the test.
researchers suggest in their report that applying the technique to new coronavirus testing could make the testing process faster and more accurate. In addition, their ultimate goal is to make the test easy to carry, thereby reducing the workload in the lab.
said they had demonstrated the importance of maintaining the level of gene expression of the virus's original RNA molecules throughout the testing process, so that more accurate analytical methods could be obtained.