Multi-comb protein PCGF5 regulates the molecular mechanism of embryonic stem cell differentiation to neuroprecursor cells.

On May 15th, Yao Hongjie, a researcher at the Guangzhou Institute of Biomedicine and Health Research of the Chinese Academy of Sciences, published the results of the study entitled PCGF5 is s a saued for neural stem stem cells in Nature Communications.
the research revealed the molecular mechanisms of polyprotein PCGF5 regulating the differentiation of embryonic stem cells into neuroprecursor cells.
epigenetic modification plays an important role in maintaining stem cell characteristics and the transformation of cell destiny.
multicomb protein as an important epigenetic modification factor was first found in fruit flies, is an important regulatory factor of epigenetic modification.
in higher animals, polycomb protein inhibition complex 1 (Polycomb Re Complex 1, or PRC1) composition is complex, according to the prC1 complex composition of different components, PRC1 complex can be divided into classic and non-classical two categories, its function performance is varied.
in an article published in Cell Stem Cell in April 2017, the team reported that non-classical polysacprotein RYBP is present in the PRC1 complex and two compounds containing the pluripotent factor OCT4, and that RYBP works together through PRC1 dependency and non-dependence to promote somatic cell reprogramming.
epigenetic factors that affect stem cell differentiation to neuroprecursor cells by screening, the team found that non-classical sub-PCGF5 of the PRC1 complex plays an important role in the process of stem cell neurodifferentiation.
their study found that knocking out PCGF5, while not affecting the dry maintenance and self-renewal of stem cells, significantly inhibited embryonic stem cell differentiation to neuroprecursor cells.
in the process of directed differentiation of stem cells to the outer nerve embryo, PCGF5 can regulate SMAD2/TGF-beta signaling pathway smad-beta by the ubiquity negative-directional control of RING1B-dependent, while the SMAD2/TGF-beta signaling pathway in the process of differentiation caused by knocking out PCGF5 is activated, which inhibits stem cell differentiation to the neuroprecursor cells.
in addition, in the process of neural differentiation of stem cells, knocking out PCGF5 makes histones H2AK119ub1 and H3K27me3 modified in the neurodifferentiation-related gene promoter region can not be effectively reduced.
and in the process of neural differentiation of stem cells, PCGF5 distribution in the whole genome not only with gene inhibition-related histone modification H2AK119ub1 and H3K27me3 co-location, more PCGF5 combined with highly expressed genes, and gene activation-related histone modification H3K27ac and H3K4me3 co-location.
the study further revealed that PCGF5 may have the function of activating the transcription of genes associated with stem cell differentiation to neuroprecursor cells.
the results of this study, PCGF5 on the one hand, by playing the function of the PRC1 complex to inhibit the SMAD2/TGF-beta signaling pathway, on the other hand, participate in the activation of nerve differentiation-related genes, and then regulate the process of stem cell differentiation to neuroprecursor cells.
this study reveals the important function of polysine PCGF5 in the process of fate transformation of embryonic stem cells, highlights that polysine not only plays an important role in gene transcription regulation inhibition, but also has the function of activating gene transcription in specific time and space, which lays a foundation for the subsequent study of the regulatory function and mechanism of key proteins in the course of the occurrence of nervous system-related diseases, and provides a way of thinking for clarifying the molecular mechanisms of the occurrence of related diseases and finding new therapeutic targets.
Yao Mingze is the first author of this paper, Yao Hongjie is the communication author of the paper.
the research was completed by Yao Hongjie's team in collaboration with Wang Huaxuan and Sun Wei of the Hong Kong Chinese University.
the research has received funding from the Chinese Academy of Sciences " organ reconstruction and manufacturing" strategic pilot science and technology project, national key research and development program, National Natural Science Foundation, Guangdong Province stem cell and tissue engineering major science and technology projects.
.