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Microdialysis HPLC method in the study of the application of fumarate ethyl pigment in the brain of awake rats local pharmacokinetics

 Last Update: 2022-09-14
 Source: Internet
 Author: User

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Fumaratel ethyl (THP) is the main active ingredient of the plant Fumarole in the family Poppyaceae, also known as tetrahydrobamastine

Microdialysis has the characteristics of continuous sampling and dynamic observation of living organisms, which is especially suitable for in vivo biochemical research

Implantation of cerebral microdialysis probe sleeves and implantation of probes

After rat anesthesia (10% chloral hydrate 3.

Collection and determination of in vivo recovery samples

After the probe is implanted, the probe is perfused with a drug-containing artificial cerebrospinal fluid with a mass concentration of ^1.

In vivo recovery rate (RR) (%) = RL (%) = (Cperfuse- Cdialysis)/Cperfuse × 100%.

The results showed that when the perfusion fluid flow rate was 1.

In this experiment, the trend of free drug concentration in awake animal tissues over time and its pharmacokinetic parameters

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