Mettl3-mediated m6A modification regulates a new mechanism for regulating the starting process of sperm stem cell differentiation and subtransfact division.

Zhou Qi and Li Wei of the Institute of Zoology of the Chinese Academy of Sciences, in collaboration with Yang Yungui of the Beijing Genomics Research Institute of the Chinese Academy of Sciences, have revealed a new mechanism for the role of Mettl3-mediated m6A (6-methyl adenine) modification in regulating the starting process of differentiation and subtransciation of sperm stem cells.
the study was published online August 15 in the journal Cell Research, under the title Mettl3-mediated m6A regulates spermatogonial and meiosis activities. The
team first used CRISPR-Cas9 technology to build a conditional knockout of Mettl3 mice in reproductive cells, revealing the male infertility and small testicular dedicate of the Mettl3 conditional knockout mice, and further studies showed that Mettl3 knockout caused abnormal differentiation of testicular sperm cells in mice, and that the reduction of the start of division was blocked.
after obtaining Mettl3 to knock out the sterile dnotypes in mice, it was found that the absence of Mettl3 led to changes in the expression of genes associated with sperm production, including sperm stem cell maintenance, differentiation, and subtransciation.
m6A-miCLIP sequencing combined with a single base resolution found that Mettl3-mediated m6A modification regulates the variable shearing of genes associated with sperm production, resulting in abnormal sperm processes.
the team found a selective mechanism for miRNA-mediated RNA methylation modification m6A methylation bits (Chen et al. Cell Stem Cell 2015), the above-mentioned Mettl3-mediated m6A regulatory sperm stem cell differentiation and reduction division starting mechanism, provides a basis for further study of the biological function of RNA methylation regulation and RNA ephemetric genetics, and provides a new direction for the study of molecular mechanisms associated with normal physiological or abnormal pathological life activities.
the research was supported by projects such as the Ministry of Science and Technology, the National Natural Science Foundation of China and the Chinese Academy of Sciences.
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