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    Home > Biochemistry News > Biotechnology News > Methods and application points for constructing stable transposable strains and their monoclonalization

    Methods and application points for constructing stable transposable strains and their monoclonalization

    • Last Update: 2022-09-14
    • Source: Internet
    • Author: User
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    Stable transposes are cell lines that obtain stable overexpression or inhibit specific genes by genetically engineered means


    ❖ Long-term study of gene function in cells of interest

    ❖ Some proteins have a very long half-life, instantaneous RNA can only interfere with expression, can not remove the expressed protein of interest, want to achieve better gene interference effect

    ❖ Need to use induced expression systems, mainly some lethal genes or need to be expressed in space and time

    ❖ Need to use cells for animal experiments, such as nude mouse tumorigenesis, etc

    ❖ The need for drug screening, the construction of recombinant proteins, the production and preparation of antibodies and the preparation of cell lines and the reporting of cell lines also need to be constructed Stable rotational strainsSecond, the advantages and necessity of


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