Metabolomics literature interpretation: deep decoding of honeycombs to regulate host behavior and nerve function

Distinct Roles of Honeybee Gut Bacteria on Host Metabolism and Neurological Processes

Published in: Microbiology Spectrum

Impact Factor: 9.

Cooperation: China Agricultural University

Baiqu Bio provides services: neurotransmitter target detection

Research background

Honeybees have a simple and host-specific gut microbiota that interacts specifically with the host and contributes to the metabolic health of their hosts, while the effects of bacterial symbiotes on host neural function remain unclear

Research results

1.

Emerging bees were colonized using six bee core gut bacteria (Figure 1A) and then a quasi-targeted metabolomics analysis was performed on hemolymphalym samples

Figure 1.

Figure 2.

2.

Since the hemolymphalytic metabolome is regulated only in bees colonized with Gi, F4 and F5, cerebral transcriptome analysis

Figure 3.

3.

Studying whether the brains of bees colonized by gut bacteria exhibited different AS events from the brains of MF bees, the results showed that multiple AS events may occur in a single gene, with intestinal members leading to different events

Genetic overlaps that indicate significant differences between bees colonized with MF and bacteria and ASD risk genes were detected from the SPARK and SFARI gene datasets

Figure 4.

4.

Enrichment analysis of differential splicing genes showed that multiple metabolic pathways, including neurotrophins, were regulated in the brains of the Gi, F4, and F5 groups (Figure 5C

Figure 5.

5.
Intestinal bacteria affect sucrose reactivity

Beak extension response (PER) is a taste-related behavior that is the basis
for bee olfactory discernment and population performance.
Measure the PER
of MF bees, conventional (CV) bees, and monofoliary Gi, F4, and F5 bees.
The sucrose sensitivity of bacterial colonization bees was significantly improved compared to the MF group (Figure 5D
).
CV bees are more sensitive to low sucrose concentrations than monosorbillers, which means the combined effect
of gut bacteria on bee behavior.
These results suggest that colonization of the normal gut microbiota or single-core gut members can affect neurotransmitter levels in the brain, which may be related
to altered olfactory sensitivity.

conclusion

In this study, by constructing bees colonized by different intestinal monobacteria, it was found that Gilliamella bacteria are associated with carbohydrate and lipid metabolism, and two Lactobacillus species mainly regulate amino acid metabolism
.
Through brain transcriptome analysis, changes in neurotransmitter transmission function are locked in, and the concentration of neurotransmitters is detected, and the changes in
neural function are verified.
The final results show that these three intestinal bacteria can affect the cognitive ability
of the host.
This study unravels the contribution of different strains to the host from the circulatory metabolome to transcriptional and neurochemical changes in the brain, highlighting the important role of the gut microbiota in bee behavior and the complex interaction
of different bee gut members with host physiology.

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Text/A-Fun Metabolomics