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Mapping DNA Target Sites of Chromatin Proteins In Vivo by Formaldehyde Crosslinking

 Last Update: 2021-01-31
 Source: Internet
 Author: User

Tags

hl 1 cells

heat 2

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The method described here is based on a technique developed to analyze the chromatin structure of the SV40 origin of replication, and also alterations in nucleosomal structures in the
hsp70
promoter of
Drosophila
after heat shock (
1
,
2
). It relies on the ability of formaldehyde to crosslink proteins and nucleic acids in living cells, from which chromatin can be isolated and protein-
DNA
interactions probed by immunoprecipitation using specific antibodies.

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