Maize (Zea mays L.)
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Last Update: 2020-11-07
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Source: Internet
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Author: User
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Agrobacterium tumefaciens
-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an
Agrobacterium
strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
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