JNNP: Synactal nucleoprotein disease as a pathological biomarker of primary REM sleep behavior disorder

Pathological studies of peripheral tissue have been conducted in patients with idiopathic rapid eye movement sleep behavior disorder (iRBD) to explore the role of potential biomarkers prior to the formation of apparent synactical nucleosis.
addition, the differences in methods make it difficult to directly compare the results of each study on outer synactal nucleoprotein disease.
to solve this problem, the researchers conducted an international study that showed that an immunosocipic chemistry (IHC) method showed 100% accuracy.
method is expected to unify the method used in gastrointestinal research, but has not been tested by other research groups.
Therefore, the purpose of this study is to assess the possibility of using upper digestive tract specimens as "pre-driven" pathological biomarkers of synth nuclear protein disease in patients with iRBD, as well as the recommended dyeing methods for verification using full-depth surgical specimens.
8 iRBD patients confirmed by polyseter sleep records and a control group that met the eligibility criteria.
After staining positive and negative control tissue using the recommended method, two adjacent segments were dyed using both the method and the dyeing method in previous gastrointestinal studies to confirm the neural structure.
clinical information and dyeing methods were evaluated by two experienced scorers.
When the two phosphate α-synaptic nucleoprotein (pAS) staining methods show inconsistent results, adjacent 5 m slices of the gastrointestinal and positive control brain (the cerebral cortitis of a patient with Louis dementia (DLB) are further dyed to check the staining quality and confirm the discovery.
the results of iRBD patients and the control group after completing the pathological assessment.
in the initial assessment, no positive results were found.
, two scorers confirmed that five subjects were positive for pAS in another.
subjects who tested positive for pAS were all iRBD patients and were statistically significant (p-0.026).
in the confirmed staining of adjacent 5 m thick slices, all slides that initially tested positive were confirmed to be pAS-positive.
the first two methods are negative.
two pAS methods tested positive on all brain slices of the DLB.
pAS positive results are usually found under the mucous membranes and in the nerve plexus of the muscle layer.
but the mucous membranes did not test positive.
the positive rates of the lower mucous membrane were 37.5% and 50.0%, respectively, in slices of 3 m and 5 sm.
pAS immune staining has high specificity and moderate sensitivity in the whole tissue of the upper digestive tract.
, because biopsy tissue is mainly mucous membrane tissue, the sensitivity of endoscopic biopsy is low, and no positive results were found in this study.
this paper also emphasizes that the consensus on the method of immune staining of gastrointestinal and nuclear cell disease needs to be validated in further large-scale studies.
Shin C, Park S, Shin JH, et al Gastric synucleinopathy as prodromal pathological biomarker in idiopathic REM sleep sleep behaviour disorder Journal of Neurology, Neurosurgery and Psysurgery Published Online First: 13 November 2020. doi: 10.1136/jnnp-2020-324743MedSci Original Source: MedSci Original Copyright Notice: All text, images and audio and video materials on this website that indicate "Source: Met Medical" or "Source: MedSci Original" are owned by Mets Medicine and are not authorized to reproduce any media, website or individual, "Source: Metz Medicine" shall be indicated at the time of authorization for reprint.
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