JEADV: Study on circulating phospholipase A2, IVD, DNA in patients with psoriasis

Autoantigens such as disintegrin and disintegrin-like thrombin-like protein motif 5 (ADAMTSL5) containing metalloprotease domain and phospholipase A2 group IVD (PLA2G4D) are involved in the pathogenesis of psoriasis
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The importance of cell-free deoxyribonucleic acid (CfDNA) as a biomarker has attracted attention in the field of dermatology, especially in psoriasis, where the concentration and circulating tumor necrosis factor-deoxyribonucleic acid level are higher than Healthy control group

Serum samples were obtained from 102 psoriasis patients (age 22-87 years; 83 men and 19 women, with or without joint involvement; presence: absent = 29:73) and 33 healthy controls
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Isolation of cfDNA from serum samples was performed as previously described and the DNA copies of psoriasis-associated autoantigens were measured using droplet digital polymerase chain reaction (ddPCR)

First, we checked for the presence of cfDNA for psoriasis-related autoantigens
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DdPCR showed that PLA2G4D DNA could be detected in normal people and patients with psoriasis (Figure 1a), but LL-37 DNA was not detected (data not shown).

Secondly, we analyzed the relationship between circulating PLA2G4D DNA copy number and clinical manifestations of psoriasis (age, gender, psoriasis area and severity index (PASI) score, presence or absence of joint involvement), and found that circulating PLA2G4D DNA level is related to Age and gender are irrelevant
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However, we did observe a weak positive correlation between circulating PLA2G4D DNA levels and PASI scores (Figure 1d)

PLA2G4D is a psoriasis antigen that is overexpressed in psoriatic skin lesions (keratinocytes, dendritic cells, macrophages and T cells)
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PLA2G4D (as a lipid antigen) specific CD1a reactive T cells produce large amounts of IL-22 and IL-17A, and these molecular mechanisms induce psoriasis inflammation

Literature source: Yamada-Kanazawa S, Mijiddorj MT, Kajihara I, Circulating phospholipase A2 group IV D DNA copies are elevated in psoriasis.