J Biotech: Milestone discovery for early detection of sepsis

Recently, in a recent study, researchers from the Institute of Computational Biotechnology at the University of Graz developed a ground-breaking approach: using endo endo-signals (biomarkers) to accurately diagnose sepsis two to three days before the first clinical symptom occurs. Based on this method, the detection can significantly improve the survival chances of patients with sepsis and reduce the adverse side effects of sepsis patients. Currently, researchers have published two papers in journal of Biotechnology, "Evaluation of host-base molecular markers for the early detection of human sepsis" and "Freedom cell-free DNA is predominantly composed of retrotransposable elements and telomeric satellite DNA", which the researchers described in detail." Our team has identified 24 biomarkers that, using newly developed classification algorithms, can detect sepsis caused by bacteria or fungi at an earlier stage, compared to the current detection methods," explains Christopher W. Sensen, director of the Institute of Computational Biotechnology at the University of Graz.For their work, bioinsyngraphers used sequencing data from anonymous plasma samples provided by a team led by Robert Krause, co-director of BioTechMed-Graz, and Peter Neumeister of the University of Graz Medical University. The samples were taken from patients diagnosed with sepsis caused by bacteria or fungi (these pathogens detected in their blood), influenza or lymphoma, and healthy people. These sequencing data form the basis for the development of algorithms for identifying markers, thus establishing a new database." This set of data can be used to distinguish between people in the early stages of sepsis and those with early clinical symptoms, as well as healthy people and patients with other diseases. In the patient group for which the markers were developed, the diagnostic accuracy was close to 90% from two days before the first clinical sign appeared to the two days after diagnosis by the diagnostic method currently in use. In blind studies of patients who were not included in the marker development, the accuracy rate was still as high as 81%. Therefore, with the help of this method, sepsis can be diagnosed earlier than other diagnostic methods.During the study, researchers also developed a new form of quantitative real-time PCR detection. PCR-based detection methods have been used clinically. Such tests are typically used to amplification the DNA or RNA of infectious pathogens in blood, plasma, or serum samples, such as bacteria or fungi that can be directly detected in patients with sepsis. However, given the wide variety of pathogens that can cause sepsis, this can only be detected on a very limited scale and is therefore highly inaccurate. On the other hand, the Graz team's newly developed detection method is mainly aimed at the body's own signals, which can represent the occurrence of sepsis of all bacteria and fungi. As a result, these signals are measured with much higher accuracy and are 2-3 days earlier than direct detection of pathogens. (Bio Valley Bioon.com)