Is there a way to prevent Marek's disease?

Paper Title: A Novel Effective and Safe Vaccine for Prevention of Marek's Disease Caused by Infection with a Very Virulent Plus (vv+) Marek's Disease Virus Marek’s disease caused by a virus infection)

Journal: Vaccines

Authors: Yifei Liao, Sanjay M.
Reddy, Owais A.
Khan, Aijun Sun and Blanca Lupiani

Posting time: 16 February 2021

DOI: 10.
3390/vaccines9020159

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https:// href="https://" target="_blank">https:// style="color:#0000ff;"> https:// style="text-indent:2em"> Marek's disease virus ( MDV) is a highly contagious alpha herpes virus, belonging to the genus Carnival virus in the Herpesviridae family
.
The virus causing Marek's disease (Marek ' S disease, MD), can cause immune suppression, neurological diseases and the rapid onset of internal organs and skin T-cell lymphoma

Experimental materials and methods

The author used a two-step Red-mediated recombination procedure to construct meq and/or vIL8 single and double deletion viruses, respectively, using immunofluorescence and immunohistochemistry techniques to determine in vitro growth viability, MDV genome copy number, and reactivate the virus in chickens.
And discussed the pathogenesis and immune protection effect
.

Results and discussion

Figure 1 shows the construction and in vitro characterization of meq or vIL8 single and double deletion viruses (686BAC-ΔMeq, 686BAC-ΔvIL8, 686BAC-ΔMeqΔvIL8)
.
RFLP results showed that the obtained pattern was completely consistent with the pattern predicted by computer analysis, and there was no accidental rearrangement in the mutant MDV genome (Figure 1B)

Figure 1.
(A) Genome structure of serotype 1 Marek's disease virus (MDV) and the location of the meq and vIL8 genes
.
(B) Genome analysis of meq and/or vIL8 deletion viruses

Figure 2.
(1) In vivo replication (A) and reactivation (B) of meq and vIL8 single and double deletion mutant viruses
.
(2) The replication of meq and vIL8 double deletion viruses in the lymphatic organs and hair follicle epithelium (FFE) of vaccinated chickens

Figure 3.
(A) Atrophy of lymphatic organs
.
(B) Survival curve of chickens vaccinated with the indicated virus or not vaccinated with the control group

Figure 4.
The protection study of meq and vIL8 double deletion viruses in MDV hen negative (Ab–) and positive (Ab +) chickens
.

in conclusion

In vitro studies have shown that the replication level of the recombinant virus with double deletion of meq and vIL8 is similar to that of the parental very virulent virus (vv+)

Journal Introduction

Vaccines (ISSN 2076-393X, IF4.
086) is an international open access journal
.
Journal Topics covered aspects of vaccines, antibodies, immune and other
.
Vaccines adopts single-blind peer review, the first review cycle is about13.
6days, and the article only takes3.
4daysfrom receipt to publication
.