Introduction to Two Methods of Cultured Cell Purification

Two major methods of cultured cell purification

The purification of cells is generally divided into two categories: natural purification and artificial purification

1.

①Overview: Due to the difference in the tolerance of epithelial cells and fibroblasts to trypsin, fibroblasts are more sensitive to trypsin, while epithelial cells are more resistant to trypsin.

②Method: Using the common digestion and passage method, inject 0.

2.

(1) Overview: After the primary culture is successful, most of the epithelial cells and fibroblasts appear at the same time and grow mixed

3.

(1) Overview: Compared with epithelial cells, fibroblasts have a faster adherence process, and most cells can usually complete the attachment process in 10-30 minutes (but not necessarily fully stretched); while most epithelial cells are in a short period of time.

(2) Method: Inoculate the cell suspension into a culture flask (preferably with serum-free culture, because epithelial cells adhere more slowly under serum-free support) and let stand for 20 minutes; observe under a microscope, see the cell part When it adheres to the wall and does not float after a little shaking, pour out the culture medium together with the unadherent cells or suck it into another culture flask; continue to culture or repeat the above operation to gradually separate the epithelial cells and fibroblasts

4.

In the same cell line, there are different cell lines, and their functions and growth characteristics are only slightly different.

The specific process is to divide the cells into single cells by the cell cloning method, grow them into clones respectively, and then test each clone to select the desired clone

5.
Medium definition method

Certain cells must be present or removed during growth or they will not grow, while other cells do the opposite
.
Cells can be purified using this technique
.
For example, the HAT medium commonly used in hybridoma technology is used to screen hybridoma cells and inhibit other cells
.

6.
Flow Cytometry Isolation

Flow cytometry can separate cells into different populations based on parameters such as cellular nucleic acid content, the content of certain substances, or the size of cellular structures
.

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