Introduction to the performance characteristics of protein chromatography and its working principle

Protein chromatography is a new liquid chromatography system for the rapid separation of peptides, nucleic acids, proteins and natural products, and its supporting columns and method templates can perform gel filtration, ion exchange, hydrophobic chromatography, reverse chromatography and affinity chromatography, suitable for applications ranging from general scientific research to drug quality testing or clinical analysis.

A variety of chromatography techniques can be manipulated for the isolation and purification of a variety of biomolecules, including native proteins, recombinant and fusion proteins, peptides, oligonucleotides, plasmids, viruses, antibiotics, alkaloids, etc.
, suitable for precise analysis and small preparation applications
such as manipulating peptide maps.

It can quickly complete the purification of biomolecular proteins, nucleic acids, peptides, etc.
, and complete the rapid development and amplification
of the entire process method from basic research in the laboratory.

It can purify the samples of biological macromolecules, the purification of known and unknown proteins, can detect, isolate and purify a variety of biological samples, and can effectively detect, purify and isolate specific biological small molecules for various samples of plants, animals, human organisms and so on
.

Five characteristics of protein chromatography purification system: 1.
Easy to operate and simple
equipment required.

Sometimes just one chromatography column is needed to work
.

Separation media – Gels can be reused
without the need for a complex regeneration process like ion exchangers.

2.
The separation effect is better and the repeatability is high
.

Highlighted is the high
sample recovery rate.

3
.
The separation conditions are relaxed.

The gel skeleton is hydrophilic, and the separation process does not involve changes in chemical bonds, so there is no adverse effect on
the activity of the isolate.

4.
Widely used
.

It is suitable for the separation and purification, desalting, concentration and analytical determination of various biochemical substances, such as peptides, hormones, proteins, polysaccharides and nucleic acids
.

The molecular weight range of the separation is also wide
.

5.
The resolution is not high, and the separation operation is slow
.

Since gel chromatography is based on the difference in molecular weight of substances, the difference in molecular weight is only manifested in the difference in flow rate, so the flow rate must be strictly grasped
during separation.

As a result, the separation operation is generally slower
.

And it is difficult to achieve good separation
for substances of similar molecular weight.

In addition, the viscosity of the sample is not required to be too high
.

Gel particles sometimes have non-specific adsorption
.

Protein chromatography is suitable for isolating and purifying proteins, enzymes, peptides, hormones, polysaccharides, nucleic acids and other substances
.

Samples whose molecular sizes differ by 25% from each other can be completely separated
as long as they pass through a single gel bed.

Using the molecular sieve properties of gels, solutions to these substances can be desalted, concentrated, deheated and decolored
.

It is ideal for
biomolecular purification.

It has the characteristics
of many functions, easy to use, and economy.

The compact design minimizes cold storage and lab workspace
.