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Digestion/decomposition of solid tissue Separation of plasma from blood to remove dead cells and debris
Cell Adhesion-Based Cell Separation Techniques Adhesion Properties of Cells · Adhesion - In vitro · In vitro
· Suspension Cells · Cancer Cells
Losing Contact Inhibition and Anchorage Dependence
.
Adhesion properties of cell culture vessels Oxidation Protein coating Coating with polylysine
Isolation of Cells by Adhesion Properties Adherent Cultures Suspension Cultures Applications, Benefits and Limitations of Adhesion-Based Isolation Specific applications include:
type | example | organize |
Mechanical homogenization | Sonication, manual pulverization, high-speed mixing | Soft tissue |
Enzymatic digestion | Collagenase, Proteinase K, Thermolysin | Soft and cartilage tissue |
acid digestion | hydrochloric acid | bone and fibrous tissue |
Table 2
.
Common methods for tissue disintegration
.
.
Common methods for tissue disintegration
.
Cell Adhesion-Based Cell Separation Techniques Adhesion Properties of Cells · Adhesion - In vitro · In vitro
· Suspension Cells · Cancer Cells
Losing Contact Inhibition and Anchorage Dependence
.
Adhesion properties of cell culture vessels Oxidation Protein coating Coating with polylysine
Isolation of Cells by Adhesion Properties Adherent Cultures Suspension Cultures Applications, Benefits and Limitations of Adhesion-Based Isolation Specific applications include: