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Figure: (a) The double-labeled fluorescent dye in the q body of the antigen fragment binds to the target antigen and is de-quenched, thereby displaying a fluorescent signal to show the intracellular target
Recent advances in imaging techniques have made it possible to visualize intracellular dynamics, which provide a better understanding
Recently, a fluorescent dye-labeled immunotransmisor called Quenchbody (Q-body) has been successfully used to detect antigens
Against this backdrop, Researchers in Japan and Singapore, led by Professor Hiroshi Ueda of Tokyo Institute of Technology (TSE) in Japan, recently reported the suitability
"Since Q-body is a site-specific and antigen-dependent imaging tool, we hypothesized that it would show antigen-dependent switchable fluorescence when interacting with the target protein, enabling accurate visualization of intracellular dynamics
The team synthesized a "double" fluorescent dye-labeled Q-body called "C11_Fab Q-body, which exhibited better sensitivity and target specificity
In contrast to traditional probes that show a continuous fluorescence signal even without p53, the Q-body probe shows a fluorescent signal
Furr's team observed that the intensity of fluorescence signal in living human colon cancer cell lines with p53 expression was 8 times
Flow cytometry shows higher Q-body immunofluorescence in p53-expressing
Professor Ueda said: "Prior art cannot accurately image
Going forward, we can expect to develop more Q bodies for visualizing several or intracellular biomarkers, opening the door
essay
Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting
