Interpretation! Scientists have developed a safer and more targeted way to transport CRISPR gene therapy!

November 29, 2020 // -- Recently, an article was published in the international magazine ACS Applied Materials . In a study published in Interfaces entitled "Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System", scientists from the University of New South Wales and others have revealed a safe and more targeted way to transport CRISPR gene therapy, which researchers say can help transport CRISPR gene therapy, and is safer and more direct than current methods.
The researchers found that lipids, commonly used in pharmacology to wrap drugs or genes, are remembered by light to release "goods" in specific parts of the body; this study is the result of researchers in cell lineages and animal models, and that further research is needed later to test and confirm the reliability of this method in humans.
so far, CRISPR gene therapy has been able to use CRISPR molecule-loaded viruses to move through the body in search of targeted cells.
although the technology has proven revolutionary, the use of viruses as vectors does not seem ideal due to its potential adverse immune responses and toxic effects.
study, researchers showed how they can use a benign carrier to carry CRISPR molecules, and they used a special method to transport them to specific parts of the body. 'The lipids we use are spherical nanostructures of lipid molecules that are very similar to cell membrane materials and can carry CRISPR molecules to the body's target site,' said Wei Deng, a
researcher.
Photo Source: Shutterstock is much safer than viruses because liposomes are the most common and sophisticated drug transport vectors; CRISPR's traditional transport vectors are virus-based, but they have their own problems because researchers have difficulty predicting how patients will react to the virus, but after finding a safer carrier to transport CRISPR cargo, the challenge for researchers is to ensure that the molecules that stitch these genes find the right target at the right time.
Unlike traditional liposome-based transport systems, the lipids used by the researchers were turned on in light and, when light hits the lipids, they were immediately destroyed and released an entire effective "load."
Using cell line and animal models, the researchers found that when lipids are induced by LED light, they release CRISPR contents that then look for genes of interest to them, which researcher Deng says activate lipids up to a centimeter below the surface of the skin.
what if the problem area is a deep tumor? The researchers say they will use X-rays to achieve the same effect in future studies.
They hope to be able to perform the X-ray triggering process of CRISPR transport in deep tissues more than a centimeter deep; previous studies have shown that lipids can be triggered by X-rays; CRISPR technology offers great hope to researchers in medical research, especially in the field of cancer research; and chemotherapy has an advantage in destroying cancer cells, but because it is not targeted, it can eventually damage normal healthy cells.
CRISPR technology provides a very promising tool for the development of new targeted, gene, and cell therapies, and the results will largely increase with the required transportation system, in which case the researchers' research provides such a system. Later
researchers said they were also interested in studying how X-rays could be used to transport CRISPR gene stitching molecules for deep cancer treatment, and that by using X-rays instead of light, researchers needed to find a suitable animal model and turn the technology into a treatment for cancers such as breast cancer, and now hope to find the right collaborators to work on the study.
() References: Yagiz Alp Aksoy, Biyao Yang, Wenjie Chen, et al. Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System, ACS applied Materials & Interfaces (2020). DOI: 10.1021/acsami.0c16380【2】Researchers show safer, more targeted way to deliver CRISPR gene therapyby Lachlan Gilbert, University of New South Wales。