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Instruction Manual for Acetylcholinesterase (AchE) Activity Assay Kit

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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5 10 models

5 ml pipette

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Acetylcholinesterase (AchE) Activity Assay Kit Instructions
Spectrophotometry 50 tubes/48 samples

Note:
Determination meaning: Determination principle: Self-provided instruments and supplies: Reagent composition and preparation: Crude enzyme liquid extraction:

Tissue: According to the ratio of tissue mass (g): extract volume (mL) of 1:5~10 (it is recommended to weigh about 0.
1g of tissue, add 1mL of extract), homogenize in ice bath, centrifuge at 8000g at 4°C for 10min, take The supernatant is to be tested
.
Bacteria and fungi: according to the number of cells (10 ⁴ ): the ratio of one volume of reagent (mL) is 500~1000:1 (it is recommended to add 1 mL of extraction solution to 5 million cells), and ultrasonically disrupt the cells in an ice bath (power 300w, ultrasonic for 3 seconds) , the interval is 7 seconds, the total time is 3 minutes); then, centrifuge at 8000g, 4 ℃ for 10 minutes, take the supernatant and put it on ice for testing
.
Serum and other liquids: direct determination
.

Measurement operation: AchE activity calculation formula:

Tissue AchE activity

⁹⁹

Bacterial, cellular AchE activity

⁴
⁴⁹

Serum AchE activity

⁹³⁶⁶

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