Inhibitory effect of rhizome extract of asparagus japonicus on proliferation of human osteosarcoma cells

Recently, researchers from the Department of Pathology, Medical College of Jishou University published a paper to explore the effect of the rhizome extract of asparagus on the proliferation of human osteosarcoma cells and its molecular mechanism It is suggested that af-a can inhibit the proliferation and growth of human osteosarcoma cells, and its mechanism may be related to inducing S-phase arrest and inhibiting the expression of COX-2 protein This article was published in the 2nd issue of Chongqing Medical Journal in 2014 MTT method was used to detect the effect of rhizome extract of asparagus japonicus on the proliferation and growth of human osteosarcoma Saos-2 cells; plate colony forming method was used to detect the effect of rhizome extract of asparagus japonicus on cell anchoring dependent growth; propidium iodide single staining flow cytometry was used to detect the change of cell cycle distribution; Western blot was used to detect the change of cyclooxygenase 2 (COX-2) protein expression level Af-a has cytotoxic effect on Saos-2 cells (IC50 value is 26.7 μ g / ml); af-a has a dose-dependent inhibitory effect on the anchoring dependent growth of Saos-2 cells (P < 0.05); af-a (30.0, 100.0 μ g / ml) treated Saos-2 cells for 48 hours, the percentage of S-phase is (43.7 ± 2.5)% and (51.9 ± 1.9)% respectively, compared with the control group (31.8 ± 4.8)% There was statistical significance (P < 0.05); different concentrations of af-a could inhibit the expression of COX-2 protein in Saos-2 cells.