Inchen flavonoids

【】Arcapillin GenkwamnCirslmaritm EupalitinCapillarisinEuparinRutin :60% 【】 This product is a light yellow to brown-yellow powder, soluble in methanol, ethanol, ethyl acetate, ice acetic acid, pyridine, dimethylmethamideidentification" take this product and ethanol to make 0.5% solution for test ingeTake the standard product of chenxerawone, inchenentone, lutein, 3' methicitin and other ethanol to make each containing 1mg/ml solution for controlTake 1.0g plus ethanol for 1.0g plus ethanol, ultrasonically extract 30 minutes, and concentrate the filter to 1 ml for controlTake the above three liquid each 6 sl, respectively, point on the same silicone G board, a point two board(1) to ethyl acetate-methanol-water (8:1:1), spray 5% aluminum tetrachloride ethanol solution, UV lamp 365nm inspection(2) to xylene-ethyl acetate-to-toxate (6:10:1) to expand, spray 5% aluminum trichloride ethanol solution, UV lamp 365nm inspectionthe above two plates, the corresponding position of the standard speckle appears the same color fluorescent spots, compared to the standard medicinal spots, the corresponding rate of 60%Dry weightlessness: No more than 3.0% (Appendix IX.G)Burning Residue: No more than 0.2% (Appendix IX.J)Heavy Metals: No more than 10ppm (Appendix IX.E)"Content Measurement" according to high-efficiency liquid chromatography (Appendix VI.D) determinationchromatography condition C18 columnmobile phase: methanol acetylene 0.5% ice acetic acid (24:19:57)flow rate: 0.7 ml/mincolumn temperature: 35 degrees Cdetection wavelength: 347nmthe standard curve to take 3'-meoxyl lutein standard smethanol is formulated to contain 5.05 ?g, 10.1 ?g per 1 ml20.2 sg30.3?g, 40.4?g solution, each divided with 20 sl intake measurement, to the peak area of the intake concentrationregression equation: Y 4.798 x 10000000X 1840 x 100000sample determination: take this product with methanol to make 0.1mg/ml solution, take 20 sl injection liquid chromatography, calculate the content of the standard curve, and calculate the total flavonoid contentfunction: liver protection, bile, lower blood pressurestorage is cool, dry and light-proof