How to use a constant temperature and humidity incubator

Constant temperature and humidity incubator is a high-precision constant temperature and humidity experimental equipment, with good stability, high degree of advantages, suitable for industrial and mining enterprises, colleges and universities, biological products, food processing, agricultural research, medical, health and epidemic prevention, environmental protection, animal husbandry research and various laboratories, for plant cultivation, environmental testing
.

The use of the constant temperature and humidity incubator step 1, check whether the constant temperature and humidity incubator identification plate is in a deactivated or running state, check the use record of the incubator, if it is normal, then hang the running logo on the incubator, you can operate
.

2.
Fill the water tank on the back of the main incubator with water
.

3.
Turn on the power switch on the incubator control panel, the indicator light on the switch is on, at this time the incubator temperature control instrument and the humidity control instrument are displayed: the temperature control instrument "PV" line (red) shows the dry bulb temperature measurement value, that is, the actual temperature in the incubator, and the "SV" line (green) shows the test temperature setting value; The humidity control instrument line "PV" (red) shows the wet bulb temperature measurement, that is, the actual temperature inside the incubator, and the "SV" line (green) shows the wet bulb temperature setpoint
.

4, temperature, humidity settings: (1) first press the function key on the temperature control instrument, at this time, the temperature value of the "SV" line will flash, and then modify the temperature setting value by pressing the "△", "▽" key of the temperature control instrument, so that the temperature value of the "SV" line reaches the temperature value required for the test, and then press "SET" to confirm
.

(2) When the temperature is set, according to the relative humidity of the test in a dry bulb temperature, check the difference corresponding to the data in the "relative humidity calculation table", and then subtract the difference in the dry bulb temperature, that is, to obtain the setting value of the humidity control instrument, the function key on the humidity instrument will be pressed, at this time, the temperature value of the "SV" line will flicker, and then modify the temperature setting value by pressing the "△", "▽" key of the temperature control instrument, so that the temperature value of the "SV" line reaches the temperature value required for the test, and then press "SET" confirmation
.

5.
Open the heating switch of the incubator, turn on the heating, the blower motor in the box runs, and the temperature control instrument begins to control the heater work
.

6.
When the dry bulb temperature measurement value reaches or approaches the dry bulb temperature setpoint, open the humidification switch, and the humidity control instrument begins to control the humidifier work
.

7.
Opening of the refrigeration machine: After the temperature and humidity setting is completed, the refrigeration machine
can be decided according to the test temperature, humidity, ambient temperature and humidity.

The general principle is that the test temperature is higher than the ambient temperature of more than 10 °C, and the test relative humidity is higher than the ambient humidity can not open the
refrigerator.

If the test temperature is lower than the ambient temperature, the refrigeration machine
is turned on when the test relative humidity is lower than the ambient humidity.

8.
After the test temperature is fully stable, if the incubator temperature display value does not reach the required temperature setting value, the temperature setting value can be slightly adjusted, and the temperature setting value
can be slightly adjusted down.

9, according to the actual needs, whether to observe the sample, decide whether to turn on the lighting, in general, do not turn on the lighting
.

10.
The interior of the incubator comes with a temperature limiting device, when the test temperature reaches about 60 ° C, the heating power supply is automatically cut off, and the over-temperature indicator light is on
at this time.

11.
After the use of the constant temperature and humidity incubator, fill in the use record
.

Precautions for the use of constant temperature and humidity incubator 1, when the incubator completes low-temperature operation, * good temperature at 60 °C to dry the treatment for about 30 minutes and then open the door of the box, so as not to affect the measurement time during the subsequent test or cause the evaporator to freeze can not do low temperature or humidity out of control phenomenon; 2.
When the constant temperature and humidity incubator is running, unless in the case of opening the incubator door as a last resort, do not open the door casually, because opening the door during operation may cause the following adverse consequences: (1) The inside of the door still maintains high temperature, and the high temperature and moisture rush out of the incubator to hurt people, causing harm to the operator; Moreover, hot air may trigger a fire alarm; (2) The damage of the switch box to the compressor during the experiment; In summary: Unless required by the test, it is not recommended that customers frequently open and close the door during operation
.

3.
When using a constant temperature and humidity incubator, it is necessary to ensure that the line is safe and grounded, and to ensure that the ground wire is isolated from the zero wire, so as to avoid electrostatic induction or damage to the incubator
.

4.
Leakage circuit breakers and over-temperature protectors, which provide testing products and the operator's personal safety protection, should be regularly inspected
in detail.

5, the installation position of the wet ball gauze should be accurate (generally hung on the thermostat near Z above the head of the water), so as to correctly measure the relative humidity
.

6.
The constant temperature and humidity incubator cannot be checked by hand touch during operation, so as to avoid electric shock or injury caused by the fan
.

7.
Non-staff can not be the constant temperature and humidity incubator machine maintenance and inspection, and when inspecting the incubator, in addition to full-time personnel, but also to have professional electricians and circuit maintenance personnel, to prevent unsuspecting personnel from energizing and closing, resulting in electric shock accidents
.

The use of constant temperature and humidity incubator - preservation of strains 1, select a constant temperature and humidity incubator for the culture of strains, as far as possible on the same incubator to complete
.

2.
The production strains stored in the sand tube or cryovial, pick a few with sterile procedures, connect them to the agar inclined medium, and incubate them at 25 °C (or higher temperature) in a constant temperature and humidity incubator for 5 to 7 days (or a longer time).
3, constant temperature and humidity incubator obtained spores need to further use a larger surface area of solid medium to obtain more spores, for the preparation of mold spores, most of the use of rice, millet and other natural media
.

The cultured mature beveled spores are made into suspensions, inoculated onto flat flasks of solid medium, and incubated at 25 to 28 °C for 14 days
.

4.
Then drain the mature flat bottle spores in a vacuum drying chamber so that the moisture drops below 10% and place them in a 4 °C refrigerator for later
.

The spore bottle prepared at one time can be used for about half a year in production, and the medium of actinomycete spores is mostly made of
pea extract, beef paste and inorganic salt and agar.

The beveled spores were inserted into the expanded flat bottle spore medium and incubated in a constant temperature and humidity incubator at 28~37°C for 5~14 days
.