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    Home > Food News > Food Articles > How much do you know about liquid chromatography

    How much do you know about liquid chromatography

    • Last Update: 2022-03-04
    • Source: Internet
    • Author: User
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    How much is a liquid chromatograph? Everyone knows that liquid chromatography has no limit to the volatilization of samples, so it has the characteristics of a wide range of mobile phases that can be selected, which makes it possible to separate and simultaneously measure substances with very similar properties, so it is widely used in biochemistry.


    Liquid chromatographs vary widely in price depending on the manufacturer, model, and instrument configuration


    The use of liquid chromatograph:

    In the analysis of polymers, adsorption chromatography is generally used to separate additives, such as azo dyes, antioxidants, surfactants, etc.


    It is used for the analysis of amino acids and proteins, and is also suitable for the separation and analysis of some inorganic ions (inorganic anions such as NO3-, SO42-, Cl-, and inorganic cations such as Na+, Ca2+, Mg2+, K+), and has a very important role


    It is suitable for the system of aqueous solution and the system of organic solvent


    High performance liquid chromatography only requires that the sample can be made into a solution, and is not limited by the volatility of the sample.


    High performance liquid chromatograph mainly includes sampling system, infusion system, separation system, detection system and data processing system


    Sampling system

    Generally, a diaphragm injection injector or a high-pressure injection chamber is used to complete the injection operation, and the injection volume is constant


    Infusion system

    The system includes three parts: high pressure pump, mobile phase reservoir and gradient meter


    separation system

    The system includes a chromatographic column, connecting pipes, and a thermostat


    The matrix in the stationary phase is composed of resin or silica gel with high mechanical strength, which have the characteristics of inertness (for example, the silicic acid gene on the surface of silica gel has been basically removed), porosity (pore size can reach 1000?) and large specific surface area.


    How much is a liquid chromatograph? To sum up, the price of liquid chromatograph is about tens of thousands of yuan each, and the expensive one is 100,000 to 200,000 yuan




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