How are samples from general test kits handled?

Tags:Assay Kit BUNSEN ELISA Kit How should samples of general assay kits be handled? Microbial Quality Peroxidase (LiP) ELISA assay kits are used to determine samples such as serum, plasma and related fluids
.

For example, suitable for testing specimens
including serum, plasma, urine, thoracoascites, lavage fluid, cerebrospinal fluid, cell culture supernatant, tissue homogenate, etc.

It is used for scientific research experiments and is not used for clinical diagnosis
.

Sample processing and requirements: 1.
Serum: Blood naturally coagulates at room temperature for 10-20 min and centrifuges for about 20 min (2000-3000 rpm
).

Collect the supernatant carefully and centrifuge
again if a precipitate occurs during preservation.

2.
Plasma: EDTA or sodium citrate should be selected as an anticoagulant according to the requirements of the specimen, mixed for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm).


Collect the supernatant carefully and centrifuge again during preservation if a pellet is formed
.

Urine: collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm
).

Collect the supernatant carefully and centrifuge again if a pellet is formed during preservation
.

Chest ascites and cerebrospinal fluid are used by reference
.

4.
Cell culture supernatant: When detecting secretory ingredients, collect
with a sterile tube.

Centrifuge for about 20 minutes (2000-3000 rpm
).

Carefully collect the supernatant
.

When detecting the composition in the cell, the cell suspension is diluted with PBS (PH7.
2-7.
4), and the cell concentration reaches about
1 million / ml.

By repeatedly freeze-thawing to allow the cells to destroy and release the ingredients within the cells
.

Centrifuge for about 20 minutes (2000-3000 rpm
).

Carefully collect the supernatant
.

If a pellet is formed during storage, it should be centrifuged
again.

5.
Tissue specimens: After cutting the specimen, weigh it
.

Add a certain amount of PBS, PH7.
4
.

Freeze quickly with liquid nitrogen for later use
.

The specimen is still maintained at a temperature
of 2-8 ° C after melting.

Add a certain amount of PBS (PH7.
4) and homogenize the specimen well by hand or with a homogenizer
.

Centrifuge for about 20 minutes (2000-3000 rpm
).

Carefully collect the supernatant
.

One serving is to be tested after aliquoting, and the rest is frozen for later use
.

6.
Extract the specimen as soon as possible after collection, the extraction is carried out according to the relevant literature, and the experiment
should be carried out as soon as possible after extraction.

If the test cannot be carried out immediately, the specimen can be stored at -20 °C, but repeated freeze-thaw should be avoided.
Samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity
.

How are samples from general test kits handled? Bunsen Biologics supplies: ELISA kits, animal serum, PCR consumables, pipette nozzles, microcentrifuge tubes, imported cryopreservation tubes, cell culture dishes, culture plates, culture bottles, imported tips, instruments and gloves, chromatography consumables, needle filters, etc
.