Guest Abstract sharing - 2018 (the third) seminar on protein modification and disease

Protein modification usually involves the addition of certain functional groups to proteins The results of modification play an important role in the regulation of protein activity, folding, stability, spatial conformation and ligand binding Common post-translational modifications of proteins include phosphorylation, acetylation, ubiquitination, glycosylation and methylation, which make the structure of proteins more complex and diverse, genetic regulation more precise and precise, function more perfect and function more specific In addition, the optimization of the pharmacokinetic properties and the improvement of the efficacy of therapeutic proteins can also be achieved by various chemical modifications Protein modification plays an important role in fine regulation of physiological activities However, many known protein modification forms, together with a large number of unknown modification forms, interact, influence and change dynamically in the multi-level cross regulation signal pathway of cells, which increases the complexity of protein modification itself The difficulty of protein modification in characterization and analysis has also brought great challenges to the research and development of related biopharmaceuticals Biovalley will hold 2018 protein modification and disease seminar in Shanghai from October 26 to 27, 2018 This conference will invite domestic experts in this field to present the latest progress in basic research, regulatory methods, detection methods and analytical technologies of protein modification from different perspectives and levels, focusing on the types and mechanisms of protein modification, the relationship with disease and drug research and development, so as to provide a cutting-edge communication platform for participants and promote basic research To practical application Guest Abstract: Chen qieshi, researcher, Kunming Institute of zoology, Chinese Academy of Sciences: Chen qieshi, researcher, doctoral director, Kunming Institute of zoology, Chinese Academy of Sciences In 1994, he received his bachelor's degree in microphysics from the school of life sciences, Nankai University; in 1999, he received his doctoral degree from Shanghai bioengineering research center and Pharmaceutical Research Institute, Chinese Academy of Sciences From 1999 to 2005, he received postdoctoral training at Virginia University and Emory University in the United States From 2006 to 2011, he served as an assistant professor and associate professor at the cell biology and cancer research center of Albany Medical College in New York Since September 2011, he has been the research team leader of tumor biology of Kunming Institute of zoology, Chinese Academy of Sciences, and the director of Key Laboratory of animal model and human disease mechanism of Chinese Academy of Sciences since 2016 In 2010, he won the "introduction of high-end scientific and technological talents" of Yunnan Province, in 2011, he won the introduction of overseas outstanding talents by Chinese Academy of Sciences (one hundred of them are preferred), in 2013, he won the "outstanding youth" of China Natural Science Foundation, in 2018, he was selected as the leader of science and technology innovation in the 10000 person plan and the special backbone talents of Chinese Academy of Sciences, and he won the special government allowance He has been engaged in breast cancer, ubiquitination, stem cells, animal models and other research for a long time, and has published more than 70 SCI papers in international journals, including J clinical investigation, nature communications, cancer research, oncogene and other papers by corresponding authors Deputy editor in chief of cancer science, editorial board member of cancer letters, JBC and zoological res Member of the Standing Committee of breast cancer of China Anti Cancer Association Topic: protein ubiquitin modification and breast cancer targeted therapy Abstract: protein ubiquitin modification regulates cell cycle, apoptosis, migration and other processes Many ubiquitin ligases, such as MDM2, VHL, BRCA1, Skp2, are closely related to the development of tumor MDM2 inhibitors have entered clinical anticancer trials In our previous studies, we found that E3 ubiquitin ligase WWP1 and scf-fbw7 promoted the ubiquitin degradation of KLF5, and that depubiquitin enzyme BaP1 could reverse this process E3 ubiquitin ligase rnf126 / bca2 promotes cancer cell cycle by promoting p21 ubiquitin degradation E3 ubiquitin ligase hectd3 promotes the survival and metastasis of breast cancer cells by modifying multiple substrate proteins These enzymes provide new targets for targeted therapy Zhan Xianquan, Professor of Xiangya Hospital of Central South University: Zhan Xianquan, Professor of Xiangya Hospital of Central South University, is mainly engaged in tumor proteomics and system biology, prediction prevention and individualized treatment target identification and precision molecular medicine research In 1999, he received a doctor's degree from West China Medical University, and in 2001, he completed his postdoctoral research in the Institute of oncology, Central South University In October 2001, Zhan Xianquan successively went to the center for Health Sciences of the University of Tennessee and the Cleveland Clinic Medical Center in the United States He was engaged in disease proteomics, mass spectrometry, biomarkers and bioinformatics as a postdoctoral researcher, project scientist, assistant professor and associate professor respectively In 2012, he returned to China full-time and served as a professor, doctoral supervisor and postdoctoral cooperation supervisor of Central South University, member, technical leader and deputy director of the technical committee of the national local joint Engineering Laboratory of anticancer drugs, deputy director of the Hunan Provincial Engineering Laboratory of structural biology and drug design, director of the cancer proteomics research laboratory, and clinical proteomics and structural biology Discipline academic leader and discipline leader, discipline leader of national key clinical specialty construction project / Key Laboratory Construction Project of oncoproteomics, discipline leader of "225" engineering medicine discipline of high-level health talents in Hunan Province, and "531" talent engineering expert of Central South University Currently, he is the national representative of frsm and EPMA, and the overseas review expert of e-cost He has published 116 research papers, participated in the compilation of 11 international academic monographs, and obtained 2 US invention patents He was invited to serve as reviewer of more than 40 international academic journals, chief editor, executive editor, deputy chief editor, guest editor, management editor of six international academic journals, and editorial board member of more than 60 international journals He was awarded the Hunan International Science and Technology Cooperation Award and the Xiaoxiang Friendship Award Speech title: revival of 2de-lc / MS in proteins and its potential for large-scale study of human proteins speech Abstract: two dimensional gel electrophyres combined with liquid chromatography / mass specification (2de-lc / MS) is a classic and conventional approach to separate and identify proteins in a protein Hover, this approach is conventionally looked upon as a seemingly “low-throughput” technique because only one to two proteins per 2D spot are often achieved; thus, it has gradually dimmed in the field of proteomics compared to seemingly “high-throughput” bottom-up non-gel approaches such as isobaric tags for relative and absolute quantification (iTRAQ), peptide tandem mass tag (TMT), stable isotope labeling of amino acids in cell culture (SILAC), and label-free With the rapid development and app lication of high-sensitivity mass spectrometry in proteomics, an average of over 50 or even hundreds of proteins can be identified in every 2D gel spot in an analysis of a complex human proteome, mostly low-abundance proteins, and 2DE-LC/MS can detect the protein species, to break through the conventional concept of 2DE-LC/MS, and assist its revival in the field of proteomics Splicing and post-translational modifications are fundamental to the proteome, and are the main factors to clarify proteoforms, which enrich the concept of the proteome The top-down and high-throughput nature of stable isotope-labeled 2DE-LC/MS for the detection, identification, Zhong Hongying Professor, School of chemistry, central China Normal University Personal profile: Zhong Hongying is currently a second-class professor in the school of chemistry, central China Normal University He is mainly engaged in the research of mass spectrometer, analytical methods and molecular structure identification methods in proteomics / metabonomics He has obtained bachelor's degree, master's degree and doctor's degree successively in the Department of chemistry of Sichuan University, the Department of chemistry of Wuhan University and the Department of chemistry of University of Alberta of Canada He was selected into the postdoctoral cross training program of roadmap, School of public health, Harvard University, and later worked in the proteome center of Rockefeller University, and also carried out cooperative research in the Department of chemistry, Stanford University The research results have been published successively as the first author or corresponding author in Nature Biotechnology, nature protocols, nature communications, analytical chemistry, analytical China Acta (featured articles in 2012 and 2018) and other international journals, and have been invited to write review articles in Journal of chromatography B and Chinese science Chemistry He has won many honors, such as the biomedical research scholar award of Wuxi apptec (2014), the first prize of excellent natural science papers of Hubei Province (2014), the Liang Shuquan prize of analytical chemistry basic research of China Chemical Society (2018), the second prize of natural science of the Ministry of Education (2017) Abstract: the significant structural difference between eukaryotic cells and prokaryotic cells lies in their developed membrane systems In addition to plasma membrane and nuclear membrane, the inner membrane system of eukaryotic cells divides the cytoplasm into different functional areas and forms various subcellular organelles with different membrane structures, including endoplasmic reticulum, Golgi body, mitochondria, etc., so as to realize the spatiotemporal regulation of biochemical reactions at different levels and ensure the orderly and normal progress of various physiological activities Post translational lipid modification of proteins refers to the covalent binding of proteins with hydrophobic lipid molecules after ribosome synthesis Among the known covalent chemical modifications, the unique physical and chemical properties of lipid molecules endow proteins with special structures and functions, It has a great influence on the membrane anchoring ability, transport and localization pathway, signal transduction and protein interaction of proteins The complexity of the function of lipid modified proteins is determined by the diverse lipid structure and binding mode These lipid modified proteins, together with other known or unknown phosphorylated modified proteins, interact in the multi-level cross regulatory signal transduction pathway of cells, It is an important way to reveal the mechanism of microbial infection, immune regulation, tumor development and so on It is of great significance to find and screen disease markers and dig new drug targets Prof Hu LIANGHAI personal profile of School of life sciences, Jilin University: Hu LIANGHAI, Professor, doctoral supervisor, School of life sciences, Jilin University He received his Bachelor of Science degree from Jilin University in July 2003 and his doctor of Science degree from Dalian Institute of chemical engineering, Chinese Academy of Sciences in January 2009 After graduation, he went to the Department of Biochemistry and cancer research center of Purdue University in the United States to engage in postdoctoral research It is mainly engaged in the research of new methods and technologies of precise medical detection based on proteomics, which provides new analytical means for solving important scientific problems in life and health research He presided over 3 NSFC projects, published more than 40 papers in the journals of angelw Chem Int ed., anal Chem., proteomics, etc., cited more than 1000 times by others, applied for 6 patents, and has been authorized 3 projects In 2012, he was selected into the "new century excellent talents support plan" of the Ministry of education, and won the excellent award of the president of the Chinese Academy of Sciences and Eli Lilly Asia excellent