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Recently, Lai Liangxue's research group from Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences published a research paper entitled Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression in Science China Life Sciences
Genetically modified pigs with high expression of foreign genes have important research and application value in the fields of biomedicine and agricultu.
During the research process, the team first adopted the strategy of double site-directed knock-in, and after cell screening with the help of two different resistance gene expression cassettes, combined with somatic cell cloning to obtain a tool for tetracycline ( Dox )-induced reporter gene expression in pigs mod.
In order to improve the efficiency of obtaining pig models of Dox -induced expression of other genes , attP sites recognized by PhiC31 recombinase were introduced on both sides of the reporter gen.
The proto-oncogene KRAS G12D mutation is one of the most common types of mutations in tumor patien.
In addition, pigs that can inducibly express exogenous genes are also a valuable genetic resource, which provides great convenience for the subsequent establishment of pig models for various purpos.
In this study, .
Paper link
Application of inducible exogenous gene expression tool in pig
