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Genetic Transformation of Cotton with a Harpin-Encoding Gene hpaXoo Confers an Enhanced Defense Response Against Verticillium dahliae Kleb

 Last Update: 2021-03-14
 Source: Internet
 Author: User

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The soil-borne fungal pathogen
Verticillium dahliae
Kleb causes Verticillium wilt in a wide range of crops including cotton (
Gossypium hirsutum
). To date, most upland cotton varieties are susceptible to
V. dahliae
and the breeding for cotton varieties with the resistance to Verticillium wilt has not been successful. Hpa1Xoo is a harpin protein from
Xanthomonas oryzae
pv. oryzae which induces the hypersensitive cell death in plants. When hpa1Xoo was transformed into the susceptible cotton line Z35 through Agrobacterium-mediated transformation, the transgenic cotton line (T-34) with an improved resistance to
Verticillium dahliae
was obtained. Here, we describe the related research approach, such as Western blot, Southern blot, immuno-gold labeling, evaluation of resistance to
Verticillium dahliae
, and how to detect the micro-hypersensitive response and oxidative burst elicited by harpin
Xoo
in plant tissue.

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